Craescu C T, Cassoly R, Galacteros F, Prehu C
Biochim Biophys Acta. 1985 Feb 14;812(3):811-5. doi: 10.1016/0005-2736(85)90276-7.
This paper reports the results of stopped-flow studies on differences in the kinetics of osmotic water transport of sickle and normal erythrocytes. The kinetics of inward osmotic water permeability are similar in sickle and normal red blood cells. In contrast, the kinetics of outward water flux are significantly (approx. 38%) decreased in sickle cells. Deoxygenation does not modify the water influx kinetics in either type of cells, but accelerates considerably the rate of water efflux in sickle cells. No significant variation of water transport kinetics was observed in density-separated cell fractions of either type. The results suggest that membrane-associated hemoglobin may decrease the outward water permeability and that in deoxygenated sickle cells the fraction of hemoglobin S near the lipid bilayer is lower than in oxygenated conditions.
本文报道了关于镰状红细胞和正常红细胞渗透水转运动力学差异的停流研究结果。镰状红细胞和正常红细胞向内的渗透水通透性动力学相似。相比之下，镰状细胞向外的水通量动力学显著降低（约38%）。脱氧不会改变任何一种类型细胞中的水流入动力学，但会显著加速镰状细胞中的水流出速率。在两种类型的密度分离细胞组分中均未观察到水转运动力学的显著变化。结果表明，与膜相关的血红蛋白可能会降低向外的水通透性，并且在脱氧的镰状细胞中，脂质双层附近的血红蛋白S比例低于氧合状态。
