Fang Hong, Wang Sa A, Medeiros L Jeffrey, Wang Wei
Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Hum Pathol. 2025 Feb;156:105711. doi: 10.1016/j.humpath.2024.105711. Epub 2024 Dec 19.
Flow cytometry immunophenotyping (FCI) is an important and indispensable tool in the diagnosis of mature B-cell lymphomas/leukemias, particularly for small fine needle aspiration and needle core biopsy specimens which are becoming increasingly popular for diagnostic purposes. FCI has several advantages. Given its multiparametric nature, FCI can analyze the expression of multiple antigens simultaneously on the same cell of interest, qualitatively and quantitively. During the diagnostic process, FCI can provide time sensitive and valuable information for triage of other ancillary studies such as immunohistochemical and molecular studies. In this review, we aim to provide common and practical approaches for using FCI in the diagnostic workup of mature B-cell neoplasms. The immunophenotypic features of common mature B-cell neoplasms as well as diagnostic challenges and pitfalls associated with FCI are also discussed.
流式细胞术免疫表型分析（FCI）是成熟B细胞淋巴瘤/白血病诊断中一项重要且不可或缺的工具，尤其适用于小细针穿刺和针芯活检标本，这些标本在诊断中越来越受欢迎。FCI具有多个优点。鉴于其多参数性质，FCI可以在同一感兴趣的细胞上同时定性和定量地分析多种抗原的表达。在诊断过程中，FCI可为其他辅助研究（如免疫组织化学和分子研究）的分类提供对时间敏感且有价值的信息。在本综述中，我们旨在提供在成熟B细胞肿瘤诊断检查中使用FCI的常见实用方法。还讨论了常见成熟B细胞肿瘤的免疫表型特征以及与FCI相关的诊断挑战和陷阱。
