Ferrer-Roda Mònica, Paramio Maria-Teresa, Vila-Beltrán Judith, Izquierdo Dolors
Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, 08193, Barcelona, Spain.
Department of Animal and Food Science, Veterinary Faculty, Autonomous University of Barcelona, 08193, Barcelona, Spain.
Theriogenology. 2025 Mar 1;234:164-173. doi: 10.1016/j.theriogenology.2024.12.015. Epub 2024 Dec 18.
Oocyte-secreted factors (OSFs), such as BMP15 and GDF9, are soluble paracrine factors that drive cumulus cell differentiation and function, sustaining oocyte competence acquisition and embryo development. This study aimed to assess the effect of BMP15 and GDF9 on IVM medium of prepubertal goat oocytes. COCs were in vitro matured in absence (control group) or presence of 100 ng/mL of BMP15, GDF9, or both. To determine cumulus-oocyte communication, transzonal projections (TZP) density at 0h, 6h, 12h and 24h of IVM were evaluated. After IVM, mitochondrial activity, intracellular ROS and glutathione (GSH) levels, the epidermal growth factor receptor (EGFR) expression in oocytes and cumulus cells, and cumulus expansion were assessed. Blastocyst production and quality were evaluated after parthenogenetic activation (PA) and IVF. IVM supplementation with BMP15 increased the TZP density during the first 6 h of culture. After IVM, BMP15 increased mitochondrial activity, EGFR expression in oocytes and cumulus cells, and cumulus expansion compared to control, but ROS and GSH levels were similar to control. BMP15 improved blastocyst production following PA (15.5 % vs 6.3 %) and the number of cells in the blastocyst inner cell mass. No differences were observed on blastocyst production or quality following IVF. IVM supplementation with GDF9 did not improve results from control group in any parameters studied. Additionally, GDF9 in combination with BMP15 only improved mitochondrial activity and cumulus expansion over control. In conclusion, IVM medium supplementation with BMP15 (100 ng/ml) improves COCs quality parameters and PA-blastocyst production and quality of prepubertal goat oocytes. However, GDF9 (100 ng/mL) did not have any beneficial effect in this study and was possibly antagonistic to BMP15.
卵母细胞分泌因子（OSFs），如骨形态发生蛋白15（BMP15）和生长分化因子9（GDF9），是可溶性旁分泌因子，可驱动卵丘细胞分化和功能，维持卵母细胞能力获得和胚胎发育。本研究旨在评估BMP15和GDF9对青春期前山羊卵母细胞体外成熟（IVM）培养基的影响。卵丘-卵母细胞复合体（COCs）在无（对照组）或有100 ng/mL BMP15、GDF9或两者存在的情况下进行体外成熟培养。为了确定卵丘-卵母细胞间的通讯，评估了IVM培养0小时、6小时、12小时和24小时时的穿卵突起（TZP）密度。IVM培养后，评估线粒体活性、细胞内活性氧（ROS）和谷胱甘肽（GSH）水平、卵母细胞和卵丘细胞中表皮生长因子受体（EGFR）的表达以及卵丘扩展情况。孤雌激活（PA）和体外受精（IVF）后评估囊胚的产生和质量。IVM培养基中添加BMP15可增加培养前6小时的TZP密度。IVM培养后，与对照组相比，BMP15增加了线粒体活性、卵母细胞和卵丘细胞中EGFR的表达以及卵丘扩展，但ROS和GSH水平与对照组相似。BMP15提高了PA后的囊胚产生率（15.5%对6.3%）以及囊胚内细胞团中的细胞数量。IVF后囊胚产生率或质量未观察到差异。IVM培养基中添加GDF9在任何研究参数上均未改善对照组的结果。此外，GDF9与BMP15联合使用仅比对照组改善了线粒体活性和卵丘扩展。总之，IVM培养基中添加100 ng/ml BMP15可改善COCs的质量参数以及青春期前山羊卵母细胞的PA-囊胚产生率和质量。然而，在本研究中100 ng/mL GDF9没有任何有益作用，并且可能与BMP15产生拮抗作用。
