Cai Xiao, Tang Liyuan, Wang Haitao, Zhang Sujun, Li Xinghe, Liu Cunjing, Zhang Xiangyun, Zhang Jianhong
Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, Key Laboratory of Cotton Biology and Genetic breeding in Huanghuaihai Semiarid Area, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, Hebei, China.
Institute of Cotton, Hebei Academy of Agriculture and Forestry Sciences, Key Laboratory of Cotton Biology and Genetic breeding in Huanghuaihai Semiarid Area, Ministry of Agriculture and Rural Affairs, Shijiazhuang 050051, Hebei, China.
Int J Biol Macromol. 2025 Mar;292:139058. doi: 10.1016/j.ijbiomac.2024.139058. Epub 2024 Dec 20.
Abiotic stress poses adverse impacts on cotton production, raising demands for a better understanding of stress-response mechanisms and developing strategies to improve plant performance to cope with stress. CYSTM (Cysteine-rich transmembrane module) is a widely distributed and conserved family in eukaryotes that performs potential functions in stress tolerance. However, CYSTM genes and their role in stress response is uncharacterized in cotton. Herein, we identified a total of 23 CYSTM genes from upland cotton. They underwent mainly segmental duplications and experienced purifying selection during evolution. Expression profiles revealed GhCYSTMs were closely related to abiotic stress response. Furthermore, GhCYSTM5_A overexpression enhanced the cold and drought tolerance of cotton, while RNAi-mediated knockdown of GhCYSTM5_A decreased stress tolerance. Transcriptome analysis revealed GhCYSTM5_A may contribute to cold and drought tolerance by regulating the expression of oxidative stress-related genes through MAPK signaling. GhCYSTM5_A, localized in the nucleus and cytoplasm interacted with a secreted cysteine-rich peptide GhGASA14. Moreover, GhGASA14 silencing rendered cotton plants vulnerable to cold and drought. These results suggested the potential functions of GhCYSTM genes in abiotic stress and a positive role of GhCYSTM5_A in cold and drought tolerance. This study sheds light on comprehensive characteristics of GhCYSTM, and provides candidate genes for genetic breeding.
非生物胁迫对棉花生产造成不利影响，这就需要更好地了解胁迫响应机制，并制定提高植物应对胁迫性能的策略。富含半胱氨酸的跨膜模块（CYSTM）是真核生物中广泛分布且保守的家族，在胁迫耐受性方面发挥潜在功能。然而，CYSTM基因及其在棉花胁迫响应中的作用尚未得到表征。在此，我们从陆地棉中总共鉴定出23个CYSTM基因。它们主要经历了片段重复，并在进化过程中受到纯化选择。表达谱显示GhCYSTMs与非生物胁迫响应密切相关。此外，GhCYSTM5_A的过表达增强了棉花的耐寒性和耐旱性，而RNA干扰介导的GhCYSTM5_A敲低则降低了胁迫耐受性。转录组分析表明，GhCYSTM5_A可能通过丝裂原活化蛋白激酶（MAPK）信号传导调节氧化应激相关基因的表达，从而有助于耐寒性和耐旱性。定位于细胞核和细胞质的GhCYSTM5_A与一种分泌的富含半胱氨酸的肽GhGASA14相互作用。此外，GhGASA14的沉默使棉花植株易受寒冷和干旱影响。这些结果表明GhCYSTM基因在非生物胁迫中的潜在功能以及GhCYSTM5_A在耐寒性和耐旱性中的积极作用。本研究揭示了GhCYSTM的综合特征，并为遗传育种提供了候选基因。
