Zhang Jielin, Chen Guopeng, Li Yuan, Zhang Jie, Zhong Liwen, Li Ling, Zhong Shihong, Gu Rui
School of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China.
BMC Plant Biol. 2024 Dec 23;24(1):1234. doi: 10.1186/s12870-024-05962-0.
Nitrogen (N) is one of the three major elements required for plant growth and development. It is of great significance to study the effects of different nitrogen application levels on the growth and root exudates of Phlomoides rotata, and can provide a theoretical basis for its scientific application of fertilizer to increase production. In this study, Phlomoides rotata were grown under different nitrogen conditions for two months. Soil and plant analyzer development (SPAD) values, bioaccumulation, root morphology, root exudate composition, nitrogen metabolism enzyme and antioxidant enzyme activity were evaluated. The results showed that compared with CK (no N fertilizer), N2 (CO(NH) 80 mg/kg) and N3 (CO(NH) 160 mg/kg) through significantly improved the activities of nitrogen metabolism enzyme nitrite reductase (NiR), glutamate dehydrogenase (GDH) and glutamine synthetase (GS), enhanced the nitrogen metabolism process, and increased the accumulation of plant soluble sugars (SS) and soluble protein (SP), thus improving Phlomoides rotata biomass yield. After 60 days of treatment, low nitrogen (N, CO(NH) 40 mg/kg) increased root length, root volume, root surface area, average root diameter, significantly increased the diversity of organic acids in root exudates, and enhanced the activity of antioxidant enzymes to adapt the nitrogen deficiency environment. This study can provide new ideas for understanding the mechanism of nitrogen tolerance in Phlomoides rotata and developing scientific fertilization management strategies for plateau plants and medicinal plants.
氮（N）是植物生长发育所需的三大主要元素之一。研究不同施氮水平对轮叶沙参生长及根系分泌物的影响具有重要意义，可为其科学施肥增产提供理论依据。本研究中，将轮叶沙参在不同氮条件下培养两个月。评估了土壤与植物养分速测仪（SPAD）值、生物积累、根系形态、根系分泌物组成、氮代谢酶和抗氧化酶活性。结果表明，与对照（不施氮肥）相比，N2（尿素80毫克/千克）和N3（尿素160毫克/千克）显著提高了氮代谢酶亚硝酸还原酶（NiR）、谷氨酸脱氢酶（GDH）和谷氨酰胺合成酶（GS）的活性，增强了氮代谢过程，增加了植物可溶性糖（SS）和可溶性蛋白（SP）的积累，从而提高了轮叶沙参的生物量产量。处理60天后，低氮（N1，尿素40毫克/千克）增加了根长、根体积、根表面积、平均根直径，显著增加了根系分泌物中有机酸的多样性，并增强了抗氧化酶活性以适应缺氮环境。本研究可为理解轮叶沙参耐氮机制及制定高原植物和药用植物科学施肥管理策略提供新思路。
