Grewal Satvir Kaur, Gill Ranjit Kaur, Virk Harpreet Kaur, Bhardwaj Rachana D
Department of Biochemistry, Punjab Agricultural University, Ludhiana, India.
Department of Biochemistry, Punjab Agricultural University, Ludhiana, India.
Plant Physiol Biochem. 2023 Mar;196:402-414. doi: 10.1016/j.plaphy.2023.01.063. Epub 2023 Feb 1.
Weed invasion causes significant yield losses in lentil. Imazethapyr (IM), a broad-spectrum herbicide inhibits the biosynthesis of branched chain amino acids necessary for plant growth. Plant growth depends upon translocation of photo-assimilates and their partitioning regulated by carbon and nitrogen metabolism. This study aimed to investigate the impact of imazethapyr spray on carbon and nitrogen metabolism in tolerant (LL1397 and LL1612) and susceptible (FLIP2004-7L and PL07) lentil genotypes during vegetative and reproductive development. Significantly higher activities of invertases and sucrose synthase (cleavage) in leaves and in podwall and seeds during early phase of development in tolerant genotypes were observed as compared to susceptible genotypes under herbicide stress that might be responsible for providing hexoses required for their growth. Activities of sucrose synthesizing enzymes, sucrose phosphate synthase and sucrose synthase (synthesis) increased significantly in podwalls and seeds of LL1397 and LL1612 genotypes during later phase of development towards maturity while the activities decreased in FLIP2004-7L and PL07 genotypes under herbicide stress. Activities of nitrate and nitrite reductase, glutamine 2-oxoglutarate aminotransferase, glutamine synthetase and glutamate dehydrogenase were increased in leaves, podwalls and seeds of LL1397 and LL1612 under herbicide stress. A proper synchronization of carbon and nitrogen metabolism in tolerant lentil genotypes during vegetative and reproductive phase might be one of the mechanisms for their recovery from herbicide stress. This first ever comprehensive information will provide a basis for future studies on the molecular mechanism of source sink relationship in lentil under herbicide stress and will be utilized in breeding programmes.
杂草入侵导致小扁豆产量大幅下降。咪唑乙烟酸（IM）是一种广谱除草剂，可抑制植物生长所需的支链氨基酸的生物合成。植物生长依赖于光合产物的转运及其由碳氮代谢调节的分配。本研究旨在调查在营养生长和生殖发育阶段，喷施咪唑乙烟酸对耐除草剂（LL1397和LL1612）和敏感（FLIP2004 - 7L和PL07）小扁豆基因型碳氮代谢的影响。与除草剂胁迫下的敏感基因型相比，在发育早期，耐除草剂基因型的叶片、荚壁和种子中的转化酶和蔗糖合酶（裂解）活性显著更高，这可能是为其生长提供所需己糖的原因。在发育后期接近成熟时，LL1397和LL1612基因型的荚壁和种子中蔗糖合成酶、蔗糖磷酸合酶和蔗糖合酶（合成）的活性显著增加，而在除草剂胁迫下，FLIP2004 - 7L和PL07基因型的这些酶活性下降。在除草剂胁迫下，LL1397和LL1612的叶片、荚壁和种子中硝酸盐和亚硝酸盐还原酶、谷氨酰胺2 - 酮戊二酸氨基转移酶、谷氨酰胺合成酶和谷氨酸脱氢酶的活性增加。耐除草剂小扁豆基因型在营养生长和生殖阶段碳氮代谢的适当同步可能是其从除草剂胁迫中恢复的机制之一。这些首次全面的信息将为未来研究除草剂胁迫下小扁豆源库关系的分子机制提供基础，并将用于育种计划。
