Huang Mingli, Ma Xiaohao, Wu Zongze, Li Jirong, Shi Yuqing, Yang Teng, Xu Jiarun, Wang Shuhan, Lv Kongpeng, Lin Yuanjing
School of Microelectronics, Southern University of Science and Technology, Shenzhen, 518055, People's Republic of China.
Department of Interventional Radiology, Shenzhen People's Hospital, Shenzhen, 518020, People's Republic of China.
Nanomicro Lett. 2024 Dec 23;17(1):92. doi: 10.1007/s40820-024-01602-2.
Ammonium level in body fluids serves as one of the critical biomarkers for healthcare, especially those relative to liver diseases. The continuous and real-time monitoring in both invasive and non-invasive manners is highly desired, while the ammonium concentrations vary largely in different body fluids. Besides, the sensing reliability based on ion-selective biosensors can be significantly interfered by potassium ions. To tackle these challenges, a flexible and biocompatible sensing patch for wireless ammonium level sensing was reported with an ultrawide linear range for universal body fluids including blood, tears, saliva, sweat and urine. The as-prepared biocompatible sensors deliver a reliable sensitivity of 58.7 mV decade in the range of 1-100 mM and a desirable selectivity coefficient of 0.11 in the interference of potassium ions, attributed to the cross-calibration within the sensors array. The sensor's biocompatibility was validated by the cell growth on the sensor surface (> 80%), hemolysis rates (< 5%), negligible cellular inflammatory responses and weight changes of the mice with implanted sensors. Such biocompatible sensors with ultrawide linear range and desirable selectivity open up new possibility of highly compatible biomarker analysis via different body fluids in versatile approaches.
体液中的铵水平是医疗保健的关键生物标志物之一，尤其是与肝脏疾病相关的标志物。人们迫切希望能够以侵入性和非侵入性方式进行连续实时监测，然而不同体液中的铵浓度差异很大。此外，基于离子选择性生物传感器的传感可靠性会受到钾离子的显著干扰。为应对这些挑战，有研究报道了一种用于无线铵水平传感的柔性且生物相容性良好的传感贴片，其对包括血液、眼泪、唾液、汗液和尿液在内的各种体液具有超宽线性范围。所制备的生物相容性传感器在1 - 100 mM范围内具有58.7 mV/十倍浓度的可靠灵敏度，在钾离子干扰下具有0.11的理想选择性系数，这归因于传感器阵列内的交叉校准。通过传感器表面细胞生长（>80%）、溶血率（<5%）、可忽略不计的细胞炎症反应以及植入传感器的小鼠体重变化，验证了该传感器的生物相容性。这种具有超宽线性范围和理想选择性的生物相容性传感器通过多种方式为通过不同体液进行高度兼容的生物标志物分析开辟了新的可能性。
