Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, PR China; Taizhou Key Laboratory of Medical Devices and Advanced Materials, Research Institute of Zhejiang University, Taizhou, 318000, PR China.
Life Sciences Institute, Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning, Guangxi, 530021, PR China.
Biosens Bioelectron. 2024 May 1;251:116136. doi: 10.1016/j.bios.2024.116136. Epub 2024 Feb 17.
Nutrition assessment is crucial for dietary guidance and prevention of malnutrition. Recent endeavors in wearable biochemical sensors have enabled real-time, in situ analysis of nutrients in sweat. However, the monitoring of riboflavin, an indispensable vitamin B involved in energy metabolism, remains challenging due to its trace level and variations in the sweat matrix. Herein, we report a wireless, battery-free, and flexible wearable biosensing system for the in situ monitoring of sweat riboflavin. Highly sensitive and selective electrochemical voltammetric detection is realized based on the synergistic effect of electrodeposited reduced graphene oxide (rGO) and platinum nanoparticles (PtNPs) with a low detection limit of 1.2 nM. The fully integrated system is capable of sweat sampling with the microfluidic patch, real-time riboflavin analysis and pH calibration with the flexible electrode array, as well as wirelessly simultaneous near field communication (NFC) energy harvesting and data transmission with the flexible circuit and a smartphone. On-body human sweat analysis demonstrates high accuracy cross-validated with gold-standard measurements, and reveals a strong correlation between sweat and urine riboflavin levels. The proposed wearable platform opens up attractive possibilities for noninvasive nutrient tracking, providing strong potential for personalized dietary guidance towards precision nutrition.
营养评估对于饮食指导和预防营养不良至关重要。最近，可穿戴式生化传感器的研究进展使得实时、原位分析汗液中的营养成分成为可能。然而，由于其痕量水平以及汗液基质的变化，监测作为能量代谢必需维生素的核黄素仍然具有挑战性。在此，我们报告了一种无线、无电池、灵活的可穿戴生物传感系统，用于原位监测汗液中的核黄素。基于电沉积还原氧化石墨烯（rGO）和铂纳米粒子（PtNPs）的协同效应，实现了高灵敏度和选择性的电化学伏安检测，检测限低至 1.2 nM。该全集成系统能够通过微流控贴片进行汗液采样，通过柔性电极阵列进行实时核黄素分析和 pH 值校准，以及通过柔性电路和智能手机进行无线近场通信（NFC）能量收集和数据传输。人体汗液的体内分析显示出与金标准测量高度吻合的准确性，并揭示了汗液和尿液核黄素水平之间的强相关性。所提出的可穿戴平台为非侵入性营养追踪开辟了诱人的可能性，为实现精准营养的个性化饮食指导提供了巨大的潜力。
