Rabei Ș O, Cârstolovean A S, Culda C A, Mihalca A D
Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Manaștur 3-5, 400372, Cluj-Napoca, Romania.
Parasitol Res. 2024 Dec 23;123(12):416. doi: 10.1007/s00436-024-08419-3.
This is the first study that targets the epidemiology of Gasterophilus spp. in slaughtered horses from Romania. Previously to our research, there were five recorded species: Gasterophilus haemorrhoidalis, Gasterophilus inermis, Gasterophilus intestinalis, Gasterophilus nasalis, and Gasterophilus pecorum with a dispersed distribution throughout the country, the data being recorded more than 73 years ago. The collection of Gasterophilus larvae was carried out from the digestive system (stomach, duodenum, and rectum) of horses in three abattoirs, monthly between January 2023 and December 2023. Following dissection, all larvae were stored in absolute ethanol until morphological and molecular identification. The larvae were counted and identified according to morphological keys. Overall, 394 horses were examined, of which 211 (53.6%) were infected with L2 or L3 of Gasterophilus spp. Only two species were found: G. intestinalis (prevalence 52.0%) and G. nasalis (14.0%). Coinfection with both species occurred in 49 horses (12.4%). In total, 9759 larvae were collected (7916 L3 and 1843 L2). Larvae were localized in the stomach (G. intestinalis) and duodenum (G. nasalis) of horses. There was a strong seasonal variation in the mean intensity and mean abundance for both G. intestinalis and G. nasalis larvae and also for coinfections (p < 0.05). There were no significant differences between prevalence and mean intensity across gender, group age, or horse colour. Our findings show that Gasterophilus infection is widespread within Romania territory, being a possible cause of illness and welfare issues in horses.
这是第一项针对罗马尼亚屠宰马匹中胃蝇属流行病学的研究。在我们的研究之前，记录有五个物种：痔状胃蝇、无刺胃蝇、肠胃蝇、鼻胃蝇和驯鹿胃蝇，其分布在全国各地，数据记录于73年多以前。2023年1月至2023年12月期间，每月从三个屠宰场的马的消化系统（胃、十二指肠和直肠）中采集胃蝇幼虫。解剖后，所有幼虫都保存在无水乙醇中，直至进行形态学和分子鉴定。根据形态学特征对幼虫进行计数和鉴定。总体而言，检查了394匹马，其中211匹（53.6%）感染了胃蝇属的L2或L3期幼虫。仅发现了两个物种：肠胃蝇（患病率52.0%）和鼻胃蝇（14.0%）。49匹马（12.4%）同时感染了这两个物种。总共收集了9759只幼虫（7916只L3期和1843只L2期）。幼虫位于马的胃（肠胃蝇）和十二指肠（鼻胃蝇）中。肠胃蝇和鼻胃蝇幼虫以及混合感染的平均强度和平均丰度均存在强烈的季节性变化（p < 0.05）。在患病率和平均强度方面，不同性别、年龄组或马的毛色之间没有显著差异。我们的研究结果表明，胃蝇感染在罗马尼亚境内广泛存在，可能是马匹患病和福利问题的一个原因。
