Moreno-Gonzalez Marta, Sierra Isabel, Kind Jop
Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) & University Medical Center Utrecht, Utrecht, The Netherlands.
Oncode Institute, Utrecht, The Netherlands.
Int J Cancer. 2024 Dec 22. doi: 10.1002/ijc.35307.
Genetic mutations are well known to influence tumorigenesis, tumor progression, treatment response and relapse, but the role of epigenetic variation in cancer progression is still largely unexplored. The lack of epigenetic understanding in cancer evolution is in part due to the limited availability of methods to examine such a heterogeneous disease. However, in the last decade the development of several single-cell methods to profile diverse chromatin features (chromatin accessibility, histone modifications, DNA methylation, etc.) has propelled the study of cancer epigenomics. In this review, we detail the current landscape of single-omic and multi-omic single-cell methods with a particular focus on the examination of histone modifications. Furthermore, we provide recommendations on both the application of these methods to cancer research and how to perform initial computational analyses. Together, this review serves as a referential framework for incorporating single-cell methods as an important tool for tumor biology.
众所周知，基因突变会影响肿瘤发生、肿瘤进展、治疗反应和复发，但表观遗传变异在癌症进展中的作用仍 largely unexplored。在癌症演变过程中，对表观遗传缺乏了解，部分原因是用于研究这种异质性疾病的方法有限。然而，在过去十年中，几种用于分析多种染色质特征（染色质可及性、组蛋白修饰、DNA甲基化等）的单细胞方法的发展推动了癌症表观基因组学的研究。在本综述中，我们详细介绍了当前单组学和多组学单细胞方法的概况，特别关注组蛋白修饰的检测。此外，我们就这些方法在癌症研究中的应用以及如何进行初步计算分析提供了建议。总之，本综述作为一个参考框架，将单细胞方法纳入肿瘤生物学的重要工具。
