Human Molecular Genetics and Biochemistry, Faculty of Medicine, Tel Aviv University, Tel Aviv 69978, Israel.
Functional Genomics and Childhood Leukemia Research Section, Cancer Research Center, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, Ramat-Gan 52621, Israel.
Nat Rev Genet. 2015 Dec;16(12):716-26. doi: 10.1038/nrg3980. Epub 2015 Oct 13.
Epigenomics is the study of the physical modifications, associations and conformations of genomic DNA sequences, with the aim of linking these with epigenetic memory, cellular identity and tissue-specific functions. While current techniques in the field are characterizing the average epigenomic features across large cell ensembles, the increasing interest in the epigenetics within complex and heterogeneous tissues is driving the development of single-cell epigenomics. We review emerging single-cell methods for capturing DNA methylation, chromatin accessibility, histone modifications, chromosome conformation and replication dynamics. Together, these techniques are rapidly becoming a powerful tool in studies of cellular plasticity and diversity, as seen in stem cells and cancer.
表观基因组学是研究基因组 DNA 序列的物理修饰、关联和构象，目的是将这些与表观遗传记忆、细胞身份和组织特异性功能联系起来。虽然该领域目前的技术正在描述大细胞群体中平均表观基因组特征，但对复杂和异质组织内表观遗传学的日益关注正在推动单细胞表观基因组学的发展。我们综述了新兴的单细胞方法，用于捕获 DNA 甲基化、染色质可及性、组蛋白修饰、染色体构象和复制动力学。这些技术共同迅速成为研究细胞可塑性和多样性的有力工具，如在干细胞和癌症中所见。
