Deng Jiahui, Zhang Ziya, Wang Xingli, Cao Yongni, Huang Huichuan, Wang Mo, Luo Qiong
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, China.
Front Plant Sci. 2024 Dec 6;15:1484214. doi: 10.3389/fpls.2024.1484214. eCollection 2024.
The rapid virulence variation of () to rice is a big challenge for rice blast control. Even though many studies have been done by scientists all over the world, the mechanism of virulence variation in remains elusive. AM16, an avirulent strain reported in our previous study, provides an excellent entry point to explore the mechanism of virulence variation in . In this study, we found that the and had specific mutations in strain AM16. The AM16 strains overexpressing or (and) allele from strain Guy11 displayed significantly increasing conidiation, functional appressorium formation, and restoring pathogenicity to rice. Moreover, we observed that the strains overexpressing had stronger conidia forming capacity than that of the strains overexpressing , while the appressorium formation rate of strains overexpressing was similar to that of strains overexpressing - , much higher than that of the strains overexpressing . Taken together, our results reveal that the natural mutation of and genes are important, but not the sole cause, for the loss of virulence in strain AM16. The functional difference between Pmk1 and Mac1 in the growth and development of was first discovered, providing new insight into the pathogenic mechanism of .
（）对水稻的毒力快速变异是稻瘟病防治面临的一大挑战。尽管世界各地的科学家已经开展了许多研究，但（）毒力变异的机制仍不清楚。AM16是我们之前研究中报道的一种无毒（）菌株，为探索（）毒力变异机制提供了一个很好的切入点。在本研究中，我们发现AM16菌株中（）和（）存在特定突变。过表达来自Guy11菌株的（）或（和）（）等位基因的AM16菌株表现出分生孢子显著增加、功能性附着胞形成以及对水稻致病性的恢复。此外，我们观察到过表达（）的菌株比分生孢子形成能力比过表达（）的菌株更强，而过表达（）的菌株的附着胞形成率与过表达（）-（）的菌株相似，远高于过表达（）的菌株。综上所述，我们的结果表明（）和（）基因的自然突变是AM16菌株毒力丧失的重要因素，但不是唯一原因。首次发现Pmk1和Mac1在（）生长发育中的功能差异，为（）致病机制提供了新的见解。
