Pathikkal Ajana, Bhaskar T Krishna, Prasanthan Aparna, Haritha P K, Puthusseri Bijesh, Rudrappa Sudha, Chauhan Vikas Singh
Plant Cell Biotechnology (PCBT) Department, Central Food Technological Research Institute (CFTRI), Mysuru, 570 020 India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201 002 India.
3 Biotech. 2025 Jan;15(1):15. doi: 10.1007/s13205-024-04170-9. Epub 2024 Dec 18.
The present study evaluated the effects of 5-methyltetrahydrofolate (5-MTHF) and aqueous extract on diabetes. An in silico docking study with select bioactive compounds showed strong binding affinities of folates with glucose metabolism-related proteins. In vitro assay showed 5-MTHF's superior inhibitory activity on alpha-amylase compared to folic acid. The protective effect of aqueous extract and 5-MTHF were validated in vivo using an STZ-induced diabetic Wistar rat model. Supplementation with extract through diet, and a higher dose of 5-MTHF through gavage effectively lowered fasting blood glucose levels and improved oral glucose tolerance and amylase content. Supplementation also countered hyperlipidemia, improved the levels of antioxidant enzymes, and reduced the inflammatory markers. Weight loss prevention, mitigation of kidney enlargement, and normalisation of the histology of the pancreas, kidney, and liver were also observed. The ameliorative effect of a higher dose of 5-MTHF was comparatively superior to aqueous extract and a corresponding higher dose of folic acid. An increase in serum folate levels on supplementation with aqueous extract suggests to be a source of bioavailable folate. The positive effect of aqueous extract suggests a potential synergistic role for folate along with its other bioactive phytochemicals. The study highlights the potential ameliorative effects of aqueous extract and 5-MTHF as a dietary supplement on diabetes and associated complications.
The online version contains supplementary material available at 10.1007/s13205-024-04170-9.
本研究评估了5-甲基四氢叶酸（5-MTHF）和水提取物对糖尿病的影响。一项对选定生物活性化合物的计算机对接研究表明，叶酸与葡萄糖代谢相关蛋白具有很强的结合亲和力。体外试验表明，与叶酸相比，5-MTHF对α-淀粉酶具有更强的抑制活性。使用链脲佐菌素诱导的糖尿病Wistar大鼠模型在体内验证了水提取物和5-MTHF的保护作用。通过饮食补充提取物，以及通过灌胃给予更高剂量的5-MTHF，可有效降低空腹血糖水平，改善口服葡萄糖耐量和淀粉酶含量。补充还可对抗高脂血症，提高抗氧化酶水平，并降低炎症标志物。还观察到预防体重减轻、减轻肾脏肿大以及胰腺、肾脏和肝脏组织学正常化。高剂量5-MTHF的改善效果相对优于水提取物和相应高剂量的叶酸。补充水提取物后血清叶酸水平升高表明其是生物可利用叶酸的一个来源。水提取物的积极作用表明叶酸与其其他生物活性植物化学物质可能具有协同作用。该研究强调了水提取物和5-MTHF作为膳食补充剂对糖尿病及相关并发症的潜在改善作用。
在线版本包含可在10.1007/s13205-024-04170-9获取的补充材料。
