Fujimaki Yoko, Kondo Kenji, Nishijima Hironobu, Kikuta Shu, Yamasoba Tatsuya
Department of Otorhinolaryngology and Head and Neck Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Department of Otolaryngology-Head and Neck Surgery, Nihon University School of Medicine, Tokyo, Japan.
Front Neurosci. 2024 Dec 5;18:1442614. doi: 10.3389/fnins.2024.1442614. eCollection 2024.
The administration of growth and neurotrophic factors has been attempted experimentally as a new therapeutic strategy for severe facial paralysis. Granulocyte colony-stimulating factor (G-CSF) has an effect on the treatment of central nervous system injuries, such as cerebral infarction and spinal cord injury. This study aimed at examining the effects of G-CSF on facial nerve regeneration in rats.
The left facial nerve of rats was either partially resected (resection group) or severed and sutured (suture group) at the main trunk outside the temporal bone. In each surgical group, saline or G-CSF was administered via the gelatin hydrogel drug delivery system. The suture group was further divided into two subgroups for the late administration of G-CSF (2 weeks after surgical treatment) or immediate administration of G-CSF after surgical treatment. Recovery of the facial nerve was assessed by the evaluation of facial movements (after 12 weeks), complex muscle action potential amplitude measurements (after 2, 4, 8, and 12 weeks), electroneurography values (after 12 weeks), and histological evaluation (comparison of myelinated axon diameters among the groups).
Recovery of the function and morphology of damaged nerves was faster in the suture groups than in the resection group. In the suture groups, recovery was faster for G-CSF-treated rats than for saline-treated rats. Furthermore, recovery was faster in the group that received G-CSF immediately after surgical treatment than in the group that received G-CSF 2 weeks later. However, the group that received G-CSF 2 weeks later also showed faster recovery than did the control group.
G-CSF effectively promoted nerve regeneration during facial nerve paralysis. Thus, G-CSF may be a potential treatment strategy for injured facial nerves as it has been safely administered in clinical treatments for hematological diseases.
作为一种治疗严重面瘫的新策略，已尝试通过实验给予生长因子和神经营养因子。粒细胞集落刺激因子（G-CSF）对治疗中枢神经系统损伤（如脑梗死和脊髓损伤）有作用。本研究旨在探讨G-CSF对大鼠面神经再生的影响。
大鼠左侧面神经在颞骨外主干处部分切除（切除组）或切断并缝合（缝合组）。在每个手术组中，通过明胶水凝胶药物递送系统给予生理盐水或G-CSF。缝合组进一步分为两个亚组，分别在手术后2周（G-CSF延迟给药）或手术后立即（G-CSF即刻给药）给予G-CSF。通过评估面部运动（术后12周）、复合肌肉动作电位幅度测量（术后2、4、8和12周）、神经电图值（术后12周）以及组织学评估（比较各组有髓轴突直径）来评估面神经的恢复情况。
缝合组受损神经的功能和形态恢复比切除组更快。在缝合组中，G-CSF治疗的大鼠比生理盐水治疗的大鼠恢复更快。此外，手术后立即接受G-CSF的组比2周后接受G-CSF的组恢复更快。然而，2周后接受G-CSF的组也比对照组恢复得更快。
G-CSF能有效促进面神经麻痹期间的神经再生。因此，由于G-CSF已在血液系统疾病的临床治疗中安全应用，它可能是一种治疗受损面神经的潜在策略。
