Wolman S R, Smith H S, Stampfer M, Hackett A J
Cancer Genet Cytogenet. 1985 Mar 1;16(1):49-64. doi: 10.1016/0165-4608(85)90077-9.
Cell cultures were derived from normal and cancerous breast tissues and from metastases by methods that selected for relatively adherent epithelial aggregates. Karyotypic analyses of first or second passage cultures yielded predominantly normal diploid cells. Nonclonal aberrations were more common in tumor-derived than in normal cultures. Three of the cultures that originated from metastases were characterized by abnormal clones. These results support observations based on DNA content, which indicate that a considerable fraction of breast cancers are composed predominantly of diploid cells. They differ greatly from chromosomal findings in long-term cultures of tumor effusions and thus emphasize the karyotypic diversity that can be found in tumors from a single tissue of origin--the breast.
通过选择相对贴壁的上皮细胞聚集体的方法，从正常和癌性乳腺组织以及转移灶中获取细胞培养物。对第一代或第二代传代培养物进行核型分析，结果显示主要为正常二倍体细胞。非克隆性畸变在肿瘤来源的培养物中比在正常培养物中更为常见。来自转移灶的三种培养物具有异常克隆特征。这些结果支持了基于DNA含量的观察结果，即相当一部分乳腺癌主要由二倍体细胞组成。它们与肿瘤积液长期培养中的染色体发现有很大差异，从而强调了在来自单一组织起源——乳腺的肿瘤中可发现的核型多样性。
