Wu Huaying, Zhang Yi, Liu Chunxiang, Tang Xiaohan, Wang Liqun, Meng Lingqi, Lu Meisong
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China.
National and Local Joint Stem Cell Research & Engineering Center for Aging Diseases, Tian Qing Stem Cell Co., Ltd, Harbin, People's Republic of China.
J Inflamm Res. 2024 Dec 17;17:11097-11109. doi: 10.2147/JIR.S483446. eCollection 2024.
To investigate the combined effects of super-active platelet lysate (sPL) and acellular amniotic membrane (AAM) in promoting endometrial repair and enhancing endometrial receptivity in rats.
The characteristics of sPL-AAM were examined through scanning electron microscopy, contact angle tester, and release experiments. We aimed to establish a rat model for endometrial injury. We divided sixty-four rats into four groups: the Injury group (Control group), the AAM group, the sPL group, and the sPL-AAM group. Our study compared the endometrial thickness, gland count, and fibrotic area recovery in rats at 6 days and 18 days post-treatment. Immunohistochemistry was utilized to assess the expressions of CD34 and ANG. Additionally, we used ELISA to detect the levels of IL-6 and TNF-α, while Western Blot was employed to compare the expressions of CK19, Integrin β3, and TGF-β1. One month after the treatment, we evaluated and compared the pregnancy recovery among the groups.
Compared to the Injury group, the sPL-AAM group demonstrated enhanced endometrial regeneration in rats at both 6 days and 18 days post-treatment, resulting in a favorable pregnancy outcome. This was achieved by promoting angiogenesis, suppressing the inflammatory response, and reducing fibrosis. The observed effects were superior to those of the sPL group alone. While sPL, when administered alone, showed some degree of endometrial restoration at 6 days post-treatment, its efficacy was diminished at 18 days post-treatment. The impact of AAM alone appeared inconspicuous compared to the injury group. This suggests that sPL serves as the primary agent in facilitating endometrial repair, while AAM functions as a carrier to extend the duration of sPL's effectiveness.
sPL-AAM can release effective cytokines, repair endometrial damage in rats, enhance endometrial receptivity, and ultimately improve pregnancy outcomes.
研究超活性血小板裂解物（sPL）与脱细胞羊膜（AAM）联合应用对促进大鼠子宫内膜修复及提高子宫内膜容受性的作用。
通过扫描电子显微镜、接触角测试仪及释放实验检测sPL-AAM的特性。建立大鼠子宫内膜损伤模型，将64只大鼠分为四组：损伤组（对照组）、AAM组、sPL组和sPL-AAM组。比较治疗后6天和18天大鼠的子宫内膜厚度、腺体数量及纤维化面积恢复情况。采用免疫组织化学法评估CD34和ANG的表达。此外，用酶联免疫吸附测定法检测白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平，用蛋白质免疫印迹法比较细胞角蛋白19（CK19）、整合素β3和转化生长因子-β1（TGF-β1）的表达。治疗1个月后，评估并比较各组的妊娠恢复情况。
与损伤组相比，sPL-AAM组在治疗后6天和18天均显示大鼠子宫内膜再生增强，妊娠结局良好，这是通过促进血管生成、抑制炎症反应和减少纤维化实现的。观察到的效果优于单独使用sPL组。单独使用sPL时，治疗后6天显示出一定程度的子宫内膜修复，但在治疗后18天其疗效减弱。与损伤组相比，单独使用AAM的影响不明显。这表明sPL是促进子宫内膜修复的主要因素，而AAM作为载体可延长sPL的有效作用时间。
sPL-AAM可释放有效细胞因子，修复大鼠子宫内膜损伤，提高子宫内膜容受性，最终改善妊娠结局。
