Chen Wei, Wang Qingyu, Xu Hongming, Xie Yuhui, Zhang Lina, Li Yao, Yan Guofeng, Ding Yiwen, Lu Shunkai, Xie Zhibo, Chen Jiarui, Xu Mengrou, Liang Xiaoben, Chen Juan, Fu Penghuai, Li Xiaoyan, Peng Liming
Department of Otolaryngology-Head and Neck Surgery, Shanghai Children's Hospital, School of Medicine Shanghai Jiao Tong University Shanghai China.
Department of Otolaryngology-Head and Neck Surgery, Huashan Hospital Fudan University Shanghai China.
Laryngoscope Investig Otolaryngol. 2024 Dec 20;9(6):e70047. doi: 10.1002/lio2.70047. eCollection 2024 Dec.
To develop a reproducible survival rabbit model for laryngotracheal stenosis (LTS).
Seventy New Zealand white (NZW) rabbits were randomly divided into experimental groups ( = 30) and a control group ( = 40). In experimental groups, a nylon brush was inserted retrograde from the tracheotomy through the subglottis and rotated until a full layer circumferential mucosal injury to cartilage exposure, assisted by fiberoptic laryngoscopy (FOL) visualization. Experimental group 1 ( = 10), rotated 10 times; group 2 ( = 20), rotated 20 times. The control group underwent tracheotomy only without nylon brush scraping. The rabbits underwent FOL at 1st, 4th, 8th, and 12th week postinjury respectively to observe the formation of LTS. They were euthanized and the larynxes and tracheas were subjected to gross and histopathological examination at 12 weeks postinjury.
The control group all survived, while five cases in experimental groups died from LTS and/or mucous plug. Histological observation showed that the control group had intact laryngotracheal mucosal epithelium without any stenosis; the experimental groups showed proliferation of fibroblasts and thickening of collagen fibers. The mean stenosis in control group was 9.31 ± 0.98%, while that in experimental group 1 was 32.78 ± 7.07% and 58.25 ± 8.96% in experimental group 2. The difference between the three groups was statistically significant ( = 47.98, < .05).
We successfully developed a reproducible survival rabbit model for LTS using a nylon brush through FOL visualization combined with tracheostomy. This model can provide a mature and stable animal model for the exploration of wound-healing pathophysiology and the effect of interventions.
NA.
建立一种可重复的喉气管狭窄（LTS）存活兔模型。
70只新西兰白兔随机分为实验组（n = 30）和对照组（n = 40）。实验组通过气管切开术将尼龙刷逆行插入声门下并旋转，直至黏膜全层环形损伤至软骨暴露，在纤维喉镜（FOL）直视辅助下进行。实验组1（n = 10）旋转10次；实验组2（n = 20）旋转20次。对照组仅行气管切开术，不进行尼龙刷刮擦。分别在损伤后第1、4、8和12周对兔子进行FOL检查，观察LTS的形成情况。在损伤后12周对兔子实施安乐死，并对喉和气管进行大体及组织病理学检查。
对照组全部存活，而实验组有5例死于LTS和/或黏液栓。组织学观察显示，对照组喉气管黏膜上皮完整，无任何狭窄；实验组显示成纤维细胞增殖和胶原纤维增厚。对照组平均狭窄率为9.31±0.98%，实验组1为32.78±7.07%，实验组2为58.25±8.96%。三组之间的差异具有统计学意义（F = 47.98，P <.05）。
我们通过FOL直视结合气管切开术，成功地利用尼龙刷建立了一种可重复的LTS存活兔模型。该模型可为探索伤口愈合病理生理学及干预效果提供成熟稳定的动物模型。
无。
