Ziegler-Heitbrock H W, Munker R, Johnson J, Petersmann I, Schmoeckel C, Riethmüller G
Cancer Res. 1985 Mar;45(3):1344-50.
Many monoclonal antibodies (MABs) have been produced against cell surface molecules of melanoma cells, and these reagents might help in the definition of stages of differentiation of the normal and the malignant cells. In an attempt to detect MAB-defined determinants that modulate with differentiation, we treated nonpigmented human melanoma cells with the tumor promotor 12-O-tetradecanoylphorbol-13-acetate (TPA) at 16 nM. Differentiation could be induced in all 4 cell lines, as evidenced by growth retardation, development of projections, and induction of melanin or of premelanosomes in the projections as detected by transmission electron microscopy. Of the 9 MAB-defined cell surface antigens, three were shown to modulate with TPA-induced differentiation, as assessed by fluorescence microscopy and fluorescence-activated cell sorter analysis. Antigens detected by MABs 15.75 and 15.95 decreased in every one of the four cells after TPA induction of differentiation. The proteoglycan defined by 225.28S increased slightly in one, showed no change in another, and decreased in the remaining two. These three MAB-defined molecules thus are linked to differentiation and might help in designing a scheme of differentiation of the melanocyte lineage.
已经产生了许多针对黑色素瘤细胞表面分子的单克隆抗体（MAB），这些试剂可能有助于确定正常细胞和恶性细胞的分化阶段。为了检测随分化而调节的MAB定义的决定簇，我们用16 nM的肿瘤促进剂12-O-十四酰佛波醇-13-乙酸酯（TPA）处理无色素的人黑色素瘤细胞。所有4种细胞系均可诱导分化，透射电子显微镜检测显示生长迟缓、突起形成以及突起中黑色素或前黑素小体的诱导可证明这一点。通过荧光显微镜和荧光激活细胞分选分析评估，在9种MAB定义的细胞表面抗原中，有3种显示随TPA诱导的分化而调节。在TPA诱导分化后，4种细胞中的每一种中，由MAB 15.75和15.95检测到的抗原均减少。由225.28S定义的蛋白聚糖在一种细胞中略有增加，在另一种细胞中无变化，在其余两种细胞中减少。因此，这三种MAB定义的分子与分化相关，可能有助于设计黑素细胞谱系的分化方案。
