Huang Louisa Z Y, Penman Rowan, Kariuki Rashad, Vaillant Pierre H A, Gharehgozlo Soroosh, Shaw Z L, Truong Vi Khanh, Vongsvivut Jitraporn, Elbourne Aaron, Caruso Rachel A
School of Science, College of STEM, RMIT University, Melbourne, Victoria 3000, Australia.
School of Engineering, College of STEM, RMIT University, Melbourne, Victoria 3000, Australia.
Nanoscale. 2025 Feb 6;17(6):3170-3188. doi: 10.1039/d4nr03238a.
Innovations in nanostructured surfaces have found a practical place in the medical area with use in implant materials for post-operative infection prevention. These textured surfaces should be dual purpose: (1) bactericidal on contact and (2) resistant to biofilm formation over prolonged periods. Here, hydrothermally etched titanium surfaces were tested against two highly antimicrobial resistant microbial species, methicillin-resistant and . Two surface types - unmodified titanium and nanostructured titanium - were incubated in a suspension of each microbial strain for 1 day and 7 days. Surface topography and cross-sectional information of the microbial cells adhered to the surfaces, along with biomass volume and live/dead rate, showed that while nanostructured titanium was able to kill microbes after 1 day of exposure, after 7 days, the rate of death becomes negligible when compared to the unmodified titanium. This suggests that as biofilms mature on a nanostructured surface, the cells that have lysed conceal the nanostructures and prime the surface for planktonic cells to adhere, decreasing the possibility of structure-induced lysis. Synchrotron macro-attenuated total reflection Fourier transform infrared (macro ATR-FTIR) micro-spectroscopy was used to elucidate the biochemical changes occurring following exposure to differing surface texture and incubation duration, providing further understanding into the effects of surface morphology on the biochemical molecules (lipids, proteins and polysaccharides) in an evolving and growing microbial colony.
纳米结构表面的创新技术在医疗领域已找到实际应用，可用于制造预防术后感染的植入材料。这些有纹理的表面应具备双重功能：（1）接触时具有杀菌作用；（2）长期抗生物膜形成。在此，对水热蚀刻钛表面针对两种高度耐抗菌的微生物物种——耐甲氧西林 和 进行了测试。将两种表面类型——未改性钛和纳米结构钛——分别在每种微生物菌株的悬浮液中孵育1天和7天。附着在表面的微生物细胞的表面形貌和横截面信息，以及生物量体积和活/死率表明，虽然纳米结构钛在暴露1天后能够杀死微生物，但7天后，与未改性钛相比，死亡率变得微不足道。这表明随着生物膜在纳米结构表面成熟，已裂解的细胞会掩盖纳米结构，并为浮游细胞附着准备好表面，从而降低结构诱导裂解的可能性。同步加速器宏观衰减全反射傅里叶变换红外光谱（宏观ATR-FTIR）显微光谱法用于阐明暴露于不同表面纹理和孵育持续时间后发生的生化变化，从而进一步了解表面形态对不断演变和生长的微生物菌落中的生化分子（脂质、蛋白质和多糖）的影响。
