Li Deze, Zheng Huiwen, Wang Xiaotong, Li Feina, Wang Heng, Chen Hao, Shen Chen, Zhao Shunying
Department of Respiratory Medicine, Beijing Children's Hospital, National Center for Children's Health, Capital Medical University, Beijing, China.
Laboratory of Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Center for Children's Health, Capital Medical University, Beijing, 100045, China.
Immunol Res. 2024 Dec 23;73(1):24. doi: 10.1007/s12026-024-09576-4.
This study aims to characterize the majority of immune cell subsets in peripheral blood mononuclear cells in children with Mycoplasma pneumoniae pneumonia (MPP) by a 21-color flow cytometry panel. Patients who met the predetermined eligibility criteria for pneumonia diagnosis were recruited for the research study. Multi-color flow cytometry was conducted on the peripheral blood mononuclear cells of each patient group, which were then subjected to dimensionality reduction and cluster analysis. In our study, the proportion of activated CD4 + T cell and naïve CD8 + T in children with MPP was higher than that of children with non-MPP, and the proportion of CD8 + T cell and central memory CD8 + T cell in MPP children was lower. Central memory CD4 + T cell and activated CD4 + T cell in the severe MPP were higher than those in the mild MPP. The highest proportions of CD8 + T cell, CD8 + Tn cell, activated CD8 + T cell, and total activated T cell were observed in the pulmonary consolidation-mucous group when compared to the pulmonary consolidation-necrosis and bronchiolitis groups. In the pulmonary consolidation-necrosis group, the proportions of central memory CD4 + T cell and T helper 17 cell were higher than those in pulmonary consolidation-mucous and bronchiolitis groups. In the bronchiolitis group, the percentages of CD4 + T cell, naïve CD4 + T cell, and T helper 2 cell were higher than those in pulmonary consolidation-mucous and the pulmonary consolidation-necrosis groups. The T lymphocyte subsets were different among various groups, offering new insights into the immune system of pediatric patients with Mycoplasma pneumoniae pneumonia.
本研究旨在通过21色流式细胞术分析，对肺炎支原体肺炎（MPP）患儿外周血单个核细胞中的大多数免疫细胞亚群进行特征描述。招募符合肺炎诊断预定入选标准的患者进行本研究。对每个患者组的外周血单个核细胞进行多色流式细胞术检测，然后进行降维和聚类分析。在我们的研究中，MPP患儿中活化CD4⁺T细胞和初始CD8⁺T细胞的比例高于非MPP患儿，而MPP患儿中CD8⁺T细胞和中枢记忆CD8⁺T细胞的比例较低。重度MPP患儿的中枢记忆CD4⁺T细胞和活化CD4⁺T细胞高于轻度MPP患儿。与肺实变-坏死组和细支气管炎组相比，肺实变-黏液组中CD8⁺T细胞、CD8⁺Tn细胞、活化CD8⁺T细胞和总活化T细胞的比例最高。在肺实变-坏死组中，中枢记忆CD4⁺T细胞和辅助性T细胞17的比例高于肺实变-黏液组和细支气管炎组。在细支气管炎组中，CD4⁺T细胞、初始CD4⁺T细胞和辅助性T细胞2的百分比高于肺实变-黏液组和肺实变-坏死组。不同组之间的T淋巴细胞亚群存在差异，这为肺炎支原体肺炎患儿的免疫系统提供了新的见解。
