Michels Arieli Carini, Scheifer Suelen Teixeira, Tiboni Fernanda, Modolo Filipe, Dos Santos Emanuela Carla, Scariot Rafaela, Aguiar Maria Cassia Ferreira, Ignácio Sergio Aparecido, de Noronha Lucia, Marins Mariana Hornung, Jham Bruno Correia, de Souza Cleber Machado, Johann Aline Cristina Batista Rodrigues
Pontifícia Universidade Católica Do Paraná. Imaculada Conceição, 1155, Prado Velho, Curitiba, PR, 80215-901, Brazil.
Universidade Federal de Santa Catarina, Eng. Agronômico Andrei Cristian Ferreira, S/N - Trindade, Florianópolis, SC, 88040-900, Brazil.
Odontology. 2024 Dec 23. doi: 10.1007/s10266-024-01044-7.
To investigate the association of NANOG polymorphisms with oral leukoplakia. In this case-control study, 68 cases of oral leukoplakia, and 21 of normal oral mucosa (control) were submitted to genotyping of tagSNPs polymorphisms: rs877716 and rs10845877 in NANOG, through real-time polymerase chain reaction (PCR). Pearson's chi-squared and Fisher's exact statistical tests were used, with a significance of 5%. For the dominant genetic model of the G allele of rs877716, the genotypes AG + GG revealed higher frequency in subjects in the oral leukoplakia group than in control subjects (75,4 and 50% respectively; p = 0,031). Subjects with this genotype were 3,063 times more likely to develop oral leukoplakia compared to subjects with AA. In the allelic genetic model, for rs10845877, the C allele was more frequent in subjects with leukoplakia than in control subjects (25 and 7, 5% respectively; p = 0, 01). There was no association found in the other genetic models. Polymorphisms in NANOG are associated with oral leukoplakia.
探讨NANOG基因多态性与口腔白斑的相关性。在这项病例对照研究中，68例口腔白斑患者和21例正常口腔黏膜（对照）通过实时聚合酶链反应（PCR）对NANOG基因中的标签单核苷酸多态性（tagSNPs）：rs877716和rs10845877进行基因分型。采用Pearson卡方检验和Fisher精确检验，显著性水平为5%。对于rs877716的G等位基因的显性遗传模型，口腔白斑组中AG + GG基因型的频率高于对照组（分别为75.4%和50%；p = 0.031）。与AA基因型的受试者相比，具有该基因型的受试者患口腔白斑的可能性高3.063倍。在等位基因遗传模型中，对于rs10845877，白斑患者中C等位基因的频率高于对照组（分别为25%和7.5%；p = 0.01）。在其他遗传模型中未发现相关性。NANOG基因多态性与口腔白斑有关。
