He Xiao, Wang Huiying, Wei Wei, Han Ziyue, Zuo Jiaqi, He Qing
School of Medical Technology, Chongqing Three Gorges Medical College, Chongqing, China.
College of Horticulture and Landscape Architecture, Southwest University, Chongqing, China.
Plant Signal Behav. 2025 Dec;20(1):2439249. doi: 10.1080/15592324.2024.2439249. Epub 2024 Dec 23.
The most damaging disease affecting citrus globally is Huanglongbing (HLB), primarily attributed to the infection by ' asiaticus' (Las). Based on comparative transcriptome data, two cellulose synthase (CESA) genes responsive to Las infection induction were screened, and one gene cloned with higher differential expression level was selected and named . we verified the interaction between CsCESA1 and citrus exopolysaccharide 2 (CsEPS2) proteins. Subcellular localization in tobacco indicated that both CsCESA1 and CsEPS2 proteins are primarily located in the nucleus and cytoplasm. RT-qPCR analysis indicated that the expression levels of and were associated with variety tolerance, tissue site, and symptom development. Furthermore, we generated and silencing plants and obtained and silencing and overexpressing hairy roots. The analysis of hormone content and gene expression also showed that and are involved in transcriptional regulation of genes involved in systemic acquired resistance (SAR) response. In conclusion, our results suggested that and could serve as potential resistance genes for HLB disease, offering insights into the plant's defense mechanisms against HLB.
全球范围内影响柑橘的最具破坏性的病害是黄龙病（HLB），主要归因于‘亚洲种’（Las）的感染。基于比较转录组数据，筛选出两个对Las感染诱导有响应的纤维素合酶（CESA）基因，并选择克隆出一个差异表达水平较高的基因并命名。我们验证了CsCESA1与柑橘胞外多糖2（CsEPS2）蛋白之间的相互作用。在烟草中的亚细胞定位表明，CsCESA1和CsEPS2蛋白主要位于细胞核和细胞质中。RT-qPCR分析表明，[此处原文缺失两个基因名称]的表达水平与品种耐受性（此处原文缺失品种耐受性相关基因名称）、组织部位和症状发展有关。此外，我们构建了[此处原文缺失两个基因名称]沉默植株，并获得了[此处原文缺失两个基因名称]沉默和过表达的毛状根（此处原文缺失两个基因名称）。激素含量和基因表达分析还表明，[此处原文缺失两个基因名称]参与了系统性获得抗性（SAR）反应相关基因的转录调控。总之，我们的结果表明，[此处原文缺失两个基因名称]可作为黄龙病潜在的抗性基因，为植物抵御黄龙病的防御机制提供了见解。
