Li Yuan, Wang Kai, Shen Dan, Liu Junze, Li Sheng, Liu Luyao, Nagaoka Kentaro, Li Chunmei
Research Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
J Anim Sci. 2025 Jan 4;103. doi: 10.1093/jas/skae388.
Lipopolysaccharide (LPS) exposure triggers pulmonary inflammation, leading to compromised lung function in broiler. As amplified by policy restrictions on antibiotic usage, seeking antibiotic alternatives has become imperative. Mogroside V (MGV) has been reported to have a beneficial role in livestock and poultry production due to its remarkable antiinflammatory effects. Despite evidence showcasing MGV's efficacy against LPS-triggered lung inflammation, its precise mechanism of action remains elusive. In this study, we transplanted normal fecal microbiota (CF), fecal microbiota modified by MGV (MF), and sterile fecal filtrate (MS) into broiler with LPS-induced pneumonia. The results showed that through fecal microbiota transplantation (FMT), transplanting MGV-induced microbial populations significantly mitigated tissue damage induced by LPS and enhanced the mRNA level of pulmonary tight junction proteins and mucoprotein (P < 0.01). The expression levels of RORα (P < 0.001), Foxp3 (P < 0.01), and PD-L1 (P < 0.01) were significantly increased in the MF group than CF group. The concentrations of IL-6 and IL-17 in broilers lung tissue of MF group were lower than those in broilers of CF group (P < 0.05). Furthermore, the concentration of TGF-β in broilers serum of MS and MF groups was higher than those in broilers of CF group (P < 0.05). Microbial community analysis demonstrated that at genus level, the harmful bacterial populations Escherichia-Shigella and Helicobacter following FMT treatment were significantly reduced in MF group (P < 0.05), potentially mediating its protective effects. Compared with CF group, valerate content and FFAR2 mRNA expression levels in MF group were significantly increased (P < 0.05). The study suggests that MGV via the gut-lung axis, attenuates Th17-mediated inflammation, offering promise as a therapeutic strategy against LPS-induced lung inflammation in chickens.
脂多糖（LPS）暴露会引发肺部炎症，导致肉鸡肺功能受损。由于对抗生素使用的政策限制不断加强，寻找抗生素替代品已变得势在必行。据报道，罗汉果甜苷V（MGV）因其显著的抗炎作用，在畜禽生产中具有有益作用。尽管有证据表明MGV对LPS引发的肺部炎症有效，但其确切作用机制仍不清楚。在本研究中，我们将正常粪便微生物群（CF）、经MGV修饰的粪便微生物群（MF）和无菌粪便滤液（MS）移植到患有LPS诱导性肺炎的肉鸡体内。结果表明，通过粪便微生物群移植（FMT），移植MGV诱导的微生物群显著减轻了LPS诱导的组织损伤，并提高了肺紧密连接蛋白和粘蛋白的mRNA水平（P<0.01）。MF组中RORα（P<0.001）、Foxp3（P<0.01）和PD-L1（P<0.01）的表达水平显著高于CF组。MF组肉鸡肺组织中IL-6和IL-17的浓度低于CF组肉鸡（P<0.05）。此外，MS组和MF组肉鸡血清中TGF-β的浓度高于CF组肉鸡（P<0.05）。微生物群落分析表明，在属水平上，FMT处理后MF组中有害细菌种群大肠杆菌-志贺氏菌属和幽门螺杆菌显著减少（P<0.05），这可能介导了其保护作用。与CF组相比，MF组中戊酸含量和FFAR2 mRNA表达水平显著升高（P<0.05）。该研究表明，MGV通过肠-肺轴减轻Th17介导的炎症，有望成为治疗鸡LPS诱导性肺炎的策略。
