Kumar Sanjay, Maskova Hana, Kuzminova Anna, Curda Paval, Doudova Lenka, Sterba Jan, Kylián Ondřej, Rego Ryan O M, Straňák Vítězslav
Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic.
Biology Centre ASCR, Institute of Parasitology, Ceske Budejovice, Czech Republic.
J Biophotonics. 2025 Feb;18(2):e202400353. doi: 10.1002/jbio.202400353. Epub 2024 Dec 23.
We report here on the development of tailored plasmonic AgNPs/C:H:N:O plasma polymer nanocomposites for the detection of the pathogenic bacterium Borrelia afzelii , with high selectivity and sensitivity. Silver (Ag) nanoparticles, generated by a gas aggregation source, are incorporated onto a C:H:N:O plasma polymer matrix, which is deposited by magnetron sputtering of a nylon 6.6. These anchored Ag nanoparticles propagate localized surface plasmon resonance (LSPR), optically responding to changes caused by immobilized pathogens near the nanoparticles. The tailored functionalization of AgNPs/C:H:N:O nanocomposite surface allows both high selectivity for the pathogen and high sensitivity with an LSPR red-shift Δλ > (4.20 ± 0.71) nm for 50 Borrelia per area 0.785 cm. The results confirmed the ability of LSPR modulation for the rapid and early detection of (not only) tested pathogens.
我们在此报告了定制的等离子体银纳米颗粒/碳氢氮氧等离子体聚合物纳米复合材料的研发情况，该材料用于检测致病性细菌阿氏疏螺旋体，具有高选择性和高灵敏度。通过气体聚集源生成的银（Ag）纳米颗粒被整合到碳氢氮氧等离子体聚合物基质上，该基质通过对尼龙6.6进行磁控溅射沉积而成。这些锚定的银纳米颗粒传播局部表面等离子体共振（LSPR），对纳米颗粒附近固定的病原体引起的变化产生光学响应。银纳米颗粒/碳氢氮氧纳米复合材料表面的定制功能化既实现了对病原体的高选择性，又具备高灵敏度，每0.785平方厘米面积上有50个阿氏疏螺旋体时，LSPR红移Δλ > (4.20 ± 0.71)纳米。结果证实了LSPR调制用于（不仅是）受试病原体快速早期检测的能力。
