Musavi Hadis, Iraie Reza, Mohammadi Maryam, Barartabar Zeinab, Yazdi Mohammad, Bagheri Abouzar, Khonakdar-Tarsi Abbas
Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Medical Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Adv Biomed Res. 2024 Nov 30;13:106. doi: 10.4103/abr.abr_456_23. eCollection 2024.
Non-alcoholic fatty liver disease is related to lipid accumulation and inflammation. Considering the role of lipin-1 and lipin-2 in fat homeostasis and inflammation, this study aimed to explore the effect of galbanic acid (Gal) and resveratrol (RSV) on alterations in the gene expression levels and protein abundance of lipin-1 and lipin-2 in HepG2 liver cells lipid-enriched with palmitate (Pal).
HepG2 cells were subjected to different amounts of Gal and RSV for 24 hours in the presence of Pal to induce lipid accumulation. The RT-PCR method was employed to assess the expression of lipin-1 and lipin-2 genes, while protein levels were evaluated by western blot analysis. Lipid accumulation was determined qualitatively and semi-quantitatively using the oil-red staining technique.
Gal treatment increased lipin-1 and lipin-2 gene expression ( < 0.05). In contrast, the groups treated with RSV did not show a substantial variance in the expression levels of the two genes ( > 0.05). In the groups treated with Gal/RSV, the intensity of lipin-2 protein bands was higher compared to the Pal group ( > 0.01); however, the intensity of lipin-1 protein bands was not significantly different ( > 0.05).
Gal, a coumarin compound, significantly increased the expression of lipin-1 and lipin-2 in HepG2 cells treated with Pal. Consequently, this research suggests gal as a novel strategy for regulating fat homeostasis in HepG2 cells.
非酒精性脂肪性肝病与脂质蓄积和炎症有关。鉴于脂联素-1和脂联素-2在脂肪稳态和炎症中的作用，本研究旨在探讨没食子酸（Gal）和白藜芦醇（RSV）对富含棕榈酸（Pal）的HepG2肝细胞中脂联素-1和脂联素-2基因表达水平及蛋白丰度变化的影响。
在Pal存在的情况下，将HepG2细胞用不同剂量的Gal和RSV处理24小时以诱导脂质蓄积。采用逆转录聚合酶链反应（RT-PCR）方法评估脂联素-1和脂联素-2基因的表达，同时通过蛋白质印迹分析评估蛋白水平。使用油红染色技术对脂质蓄积进行定性和半定量测定。
Gal处理增加了脂联素-1和脂联素-2基因的表达（<0.05）。相比之下，用RSV处理的组在这两个基因的表达水平上没有显示出显著差异（>0.05）。在Gal/RSV处理组中，脂联素-2蛋白条带的强度高于Pal组（>0.01）；然而，脂联素-1蛋白条带的强度没有显著差异（>0.05）。
香豆素类化合物Gal显著增加了用Pal处理的HepG2细胞中脂联素-1和脂联素-2的表达。因此，本研究表明Gal是调节HepG2细胞脂肪稳态的一种新策略。
