Ito Akane, Isamoto Kazuto, Morishita Yuhei, Tanimizu Masaharu
Department of Earth Resources Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Department of Applied Chemistry for Environment, School of Science and Technology, Kwansei Gakuin University, Gakuen-Uegahara-1, Sanda, Hyogo 669-1330, Japan.
Mass Spectrom (Tokyo). 2024;13(1):A0161. doi: 10.5702/massspectrometry.A0161. Epub 2024 Dec 18.
A simple and rapid analytical method was developed for the simultaneous determination of two chromium species, Cr(III) and Cr(VI), in the environmental waters by high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). This study incorporated a chelating pretreatment with 2,6-pyridinedicarboxylic acid (PDCA) to convert Cr(III) species into a stable Cr(III)-PDCA anion complex, which was then separated from Cr(VI) oxyanion using an anion exchange column. Building on the fundamental analytical approach proposed by Shigeta . (doi: 10.2116/analsci.18P012), the mobile phase was optimized to ensure stability for ICP-MS detection, avoiding nonvolatile salts. Chromium species and chloride ions were effectively separated within 6 minutes at a flow rate of 0.6 mL min with the optimized mobile phase, which consisted of 50 mmol L ammonium acetate (pH 6.80) and 2 mmol L PDCA. The detection limits were 0.18 μg L and 0.09 μg L for Cr(III) and Cr(VI), respectively, at 52 under He collision mode.
建立了一种简单快速的分析方法，用于通过高效液相色谱-电感耦合等离子体质谱法（HPLC-ICP-MS）同时测定环境水体中的两种铬形态，即Cr(III)和Cr(VI)。本研究采用2,6-吡啶二甲酸（PDCA）进行螯合预处理，将Cr(III)形态转化为稳定的Cr(III)-PDCA阴离子络合物，然后使用阴离子交换柱将其与Cr(VI)含氧阴离子分离。基于Shigeta提出的基本分析方法（doi: 10.2116/analsci.18P012），对流动相进行了优化，以确保ICP-MS检测的稳定性，避免使用非挥发性盐。使用由50 mmol/L醋酸铵（pH 6.80）和2 mmol/L PDCA组成的优化流动相，在流速为0.6 mL/min的条件下，6分钟内可有效分离铬形态和氯离子。在He碰撞模式下，52时Cr(III)和Cr(VI)的检测限分别为0.18 μg/L和0.09 μg/L。
