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油棕叶斑病抗性的分子基础。

Molecular basis of resistance to leaf spot disease in oil palm.

作者信息

Wibowo Cahyo S, Susilo Ricki, Ernawan Reza, Apriyanto Ardha, Alshaharni Mohammed O, Smith Graham R, Gatehouse Angharad M R, Edwards Martin G

机构信息

School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom.

Research and Development, PT Astra Agro Lestari Tbk (Astra Agro), Jakarta, Indonesia.

出版信息

Front Plant Sci. 2024 Dec 9;15:1458346. doi: 10.3389/fpls.2024.1458346. eCollection 2024.

DOI:10.3389/fpls.2024.1458346
PMID:39717734
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11663676/
Abstract

INTRODUCTION

Leaf spot disease caused by the fungal pathogen is one of the most common diseases found in oil palm () nurseries in South East Asia, and is most prevalent at the seedling stage. Severe infections result in localized necrotic regions of leaves that rapidly spread within nurseries leading to poor quality seedlings and high economic losses.

METHODS

To understand the molecular mechanisms of this plant-pathogen interaction, RNA-Seq was used to elucidate the transcriptomes of three oil palm genotypes with contrasting pathogen responses (G10 and G12, resistant and G14, susceptible) following infection with spores. Transcriptomes were obtained from Illumina NovaSeq 6000 sequencing of mRNA at four different time points (day 0, before treatment; day 1, 7, and 21 post treatment).

RESULTS AND DISCUSSION

Analysis of differentially expressed gene (DEG) profiles in these three genotypes provided an overview of the genes involved in the plant defence. Genes involved in disease resistance, phytohormone biosynthesis, gene regulation (transcription factors), and those encoding proteins associated with cell wall hardening were identified and likely contribute to the resistance of oil palm to . Such genes represent good candidates for targets to enhance oil palm productivity and resilience through molecular breeding approaches.

摘要

引言

由真菌病原体引起的叶斑病是东南亚油棕苗圃中最常见的病害之一，在幼苗期最为普遍。严重感染会导致叶片出现局部坏死区域，这些区域会在苗圃内迅速蔓延，导致幼苗质量下降和经济损失惨重。

方法

为了解这种植物-病原体相互作用的分子机制，利用RNA测序技术来阐明三种对病原体反应不同的油棕基因型（G10和G12，抗病；G14，感病）在接种孢子后的转录组。转录组是通过对四个不同时间点（处理前第0天；处理后第1天、第7天和第21天）的mRNA进行Illumina NovaSeq 6000测序获得的。

结果与讨论

对这三种基因型中差异表达基因（DEG）图谱的分析提供了参与植物防御的基因概况。鉴定出了与抗病性、植物激素生物合成、基因调控（转录因子）以及编码与细胞壁硬化相关蛋白质的基因，这些基因可能有助于油棕对该病原体的抗性。此类基因是通过分子育种方法提高油棕生产力和恢复力的良好靶点候选基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87dc/11663676/df18c643c894/fpls-15-1458346-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87dc/11663676/54fe873686f8/fpls-15-1458346-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87dc/11663676/510fba1bf57b/fpls-15-1458346-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87dc/11663676/df18c643c894/fpls-15-1458346-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87dc/11663676/54fe873686f8/fpls-15-1458346-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87dc/11663676/510fba1bf57b/fpls-15-1458346-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87dc/11663676/df18c643c894/fpls-15-1458346-g003.jpg

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