Unakal Chandrashekhar, Blake Lemar Wayne, Farfan Gerard, Justiz-Vaillant Angel, Pandit Bijay Raj, Akpaka Patrick Eberechi
Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
The Endoscopy Centre, Woodbrook, Port of Spain, Trinidad and Tobago.
IJID Reg. 2024 Nov 22;14:100498. doi: 10.1016/j.ijregi.2024.100498. eCollection 2025 Mar.
To delineate and understand the genetic variations among strains from Trinidad and Tobago associated with gastric diseases.
One hundred (n = 100) patients who routinely presented with clinical features suggestive of peptic disease were enrolled in the study and underwent gastroscopy procedures. Biopsy specimens were analyzed using serological and molecular methods. Amplification of the variable vacuolating cytotoxin gene A (A), urease C (C), and 16S ribosomal RNA regions was performed by polymerase chain reaction; and then followed by sequencing and alignment. Phylogenetic analysis and DNA fragment alignment were carried out using DNA MEGA 7 version 7.
Overall, 70 of the patients tested positive for infection using an enzyme immune assay. All were 16S ribosomal RNA positive, with 8.6% (6/70) testing positive for S1 and 91.4% (64/70) for S2. The most prevalent A allelic combination was S2M1, with 87.1% (61/70) cases. All S1M1 strains were C-positive, while S1M2 strains were C-negative.
strain variations are seen in the Trinidad and Tobago isolates and are most closely related to the ones from the USA, India, and Indonesia. However, the factors contributing to the emergence of S2/M1 being more prevalent will need to be further investigated.
描绘并了解特立尼达和多巴哥与胃部疾病相关菌株的基因变异情况。
100名经常表现出消化性疾病临床特征的患者纳入本研究并接受胃镜检查。活检标本采用血清学和分子方法进行分析。通过聚合酶链反应对可变空泡毒素基因A（A）、脲酶C（C）和16S核糖体RNA区域进行扩增；然后进行测序和比对。使用DNA MEGA 7版本7进行系统发育分析和DNA片段比对。
总体而言，使用酶免疫测定法检测，70名患者感染呈阳性。所有患者16S核糖体RNA均为阳性，其中8.6%（6/70）的S1检测呈阳性，91.4%（64/70）的S2检测呈阳性。最常见的A等位基因组合是S2M1，占87.1%（61/70）的病例。所有S1M1菌株C呈阳性，而S1M2菌株C呈阴性。
在特立尼达和多巴哥分离株中观察到菌株变异，且与来自美国、印度和印度尼西亚的菌株关系最为密切。然而，导致S2/M1更为普遍出现的因素需要进一步研究。
