Ghotaslou Reza, Milani Morteza, Akhi Mohammad Taghi, Nahaei Mohammad Reza, Hasani Alka, Hejazi Mohammad Saeid, Meshkini Mohammad
Liver and Gastroenterology Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Adv Pharm Bull. 2013;3(1):57-62. doi: 10.5681/apb.2013.010. Epub 2013 Feb 7.
The purpose of this research was to analyze cagA and vacA genotypes status in H. pylori isolates and relationship with clinical outcomes.
Gastric biopsy specimens were cultured for H. pylori isolation and cagA and vacA genes were detected in these isolates. Data were collected and the results were analyzed using χ2 and Fishers exact tests by SPSS software version. 16.
Of the total 115 H. pylori isolates, 79 (68.7 %) were cagA positive and 82 (71.3%) of isolates contained the s1 allele which 33 (28.7%) were subtype s2. s1m2 was the most frequent vacA allelic combination in the H. pylori isolates examined (63 cases), followed by s2m2 (31 cases), s1m1 (19 cases) and s2m1 (2 case). Strains cagA positive were more frequent in peptic ulcer diseases patients than non ulcer diseases patients, as 47 (59.5%) and 32 (40.5%), while cagA negative were low, as 15 (41.7%) and 21 (58.3%), respectively.
We found that the cagA and vacA status were not related to clinical outcomes in this area. Overall, in the present study, vacA s1/m2, cagA-positive strains were predominant irrespective of clinical outcome, but s2/m1 was rare.
本研究旨在分析幽门螺杆菌分离株中cagA和vacA基因型状况及其与临床结局的关系。
对胃活检标本进行培养以分离幽门螺杆菌，并检测这些分离株中的cagA和vacA基因。收集数据并使用SPSS 16版软件通过χ2检验和Fisher精确检验进行结果分析。
在总共115株幽门螺杆菌分离株中，79株（68.7%）cagA呈阳性，82株（71.3%）分离株含有s1等位基因，其中33株（28.7%）为s2亚型。s1m2是所检测的幽门螺杆菌分离株中最常见的vacA等位基因组合（63例），其次是s2m2（31例）、s1m1（19例）和s2m1（2例）。消化性溃疡疾病患者中cagA阳性菌株比非溃疡疾病患者更常见，分别为47株（59.5%）和32株（40.5%），而cagA阴性菌株则较低，分别为15株（41.7%）和21株（58.3%）。
我们发现该地区cagA和vacA状况与临床结局无关。总体而言，在本研究中，无论临床结局如何，vacA s1/m2、cagA阳性菌株均占主导地位，但s2/m1罕见。
