Topalović Dijana, Živković Lada, Borozan Sunčica, Santibanez Juan F, Spremo-Potparević Biljana
1University of Belgrade Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia.
2University of Belgrade Faculty of Veterinary Medicine, Department of Chemistry, Belgrade, Serbia.
Arh Hig Rada Toksikol. 2024 Dec 29;75(4):297-302. doi: 10.2478/aiht-2024-75-3915. eCollection 2024 Dec 1.
Medicinal mushroom extracts, i.e. their dried biomass, have long been known as sources of bioactive compounds with positive effects on the human health. The antioxidant, antigenotoxic, antiviral, and immunomodulatory properties of the commercially available extracts auct. non Murrill (AB), (Berk.) Sacc. (CS), and Immune Assist (IA) have already been documented. This study, studied the influence of these three mushrooms on the viability of cell lines MCF-7, MDA-MB-231, and HS-5. The cytotoxicity of AB, CS, and IA at different concentrations (25, 50, 100, 200, 400 and 800 μg/mL) was evaluated using the MTT assay. The results showed that AB was the most effective and induced cytotoxicity in both cancer cell lines, with IC values of 96.7 μg/mL for MCF-7 and 368.4 μg/mL for MDA-MB-231. After treatment with CS and IA, the half-maximal inhibitory concentration was reached only in MDA- MB-231 cells (IC=613 μg/mL for CS and 343.3 μg/mL for IA). We have shown here that AB, CS and IA can suppress the growth of MCF-7 and MDA-MB-231 cell lines, while affecting the survival of healthy HS-5 cells to a much lesser extent. Our results suggested that AB, CS and IA are promising natural sources with potential anticancer activity.
药用蘑菇提取物，即其干燥的生物质，长期以来一直被认为是对人体健康有积极影响的生物活性化合物的来源。市售提取物auct. non Murrill（AB）、(Berk.) Sacc.（CS）和免疫辅助剂（IA）的抗氧化、抗遗传毒性、抗病毒和免疫调节特性已经得到了记录。本研究考察了这三种蘑菇对MCF-7、MDA-MB-231和HS-5细胞系活力的影响。使用MTT法评估了不同浓度（25、50、100、200、400和800μg/mL）的AB、CS和IA的细胞毒性。结果表明，AB在两种癌细胞系中最有效且诱导了细胞毒性，MCF-7的IC值为96.7μg/mL，MDA-MB-231的IC值为368.4μg/mL。用CS和IA处理后，仅在MDA-MB-231细胞中达到半数最大抑制浓度（CS的IC = 613μg/mL，IA的IC = 343.3μg/mL）。我们在此表明，AB、CS和IA可以抑制MCF-7和MDA-MB-231细胞系的生长，同时对健康的HS-5细胞的存活影响要小得多。我们的结果表明，AB、CS和IA是有潜力的具有潜在抗癌活性的天然来源。
