Jin Hui, Yoon Hyeon-Jeong, Jiang Enying, Liu Jingting, Yoon Hee Su, Choi Ji Suk, Moon Jayoung, Qi Hong, Yoon Kyung Chul
Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, Korea.
Department of Biomedical Sciences and Centers for Creative Biomedical Scientists at Chonnam National University, Gwangju, Korea.
J Ocul Pharmacol Ther. 2025 Apr;41(3):141-149. doi: 10.1089/jop.2024.0121. Epub 2024 Dec 24.
To evaluate the efficacy of human placental extract (HPE) eye drops compared to that of carboxymethylcellulose (CMC) and human peripheral blood serum (HPBS) eye drops in a mouse model of experimental dry eye (EDE) and corneal alkali burns. EDE and alkali burn models were induced in C57BL/6 mice using desiccating stress and NaOH, respectively. In both the EDE and alkali burn models, treatment groups received CMC, HPBS, or HPE eye drops. In EDE model, tear volume, tear break-up time (TBUT), and total corneal fluorescein staining score (CFSS) were measured. ROS were detected with 2',7'-dichlorodihydrofluorescein diacetate. Conjunctiva goblet cells were identified by periodic acid-Schiff staining, and corneal epithelial apoptosis was detected by TUNEL assay. In alkali burn model, the area and diameter of epithelial defects were assessed in each group. In the EDE model, tear volume, CFSS, and epithelial apoptosis were significantly improved in all treatment groups. Compared to the CMC group, the HPE group showed a better improvement in the production of tear volume, TBUT, CFSS, ROS, and conjunctiva goblet cell density. There were no significant differences in parameters between the HPBS and HPE groups except for TBUT at 14 days. In the alkali burn model, the HPE group had a smaller area compared to the control and CMC groups and a shorter diameter compared to the control group. HPE eye drops were as effective as HPBS eye drops in improving the clinical signs and ocular surface oxidative damage of EDE and in promoting corneal epithelialization after alkali burn.
在实验性干眼（EDE）和角膜碱烧伤小鼠模型中，评估人胎盘提取物（HPE）滴眼液与羧甲基纤维素（CMC）和人外周血血清（HPBS）滴眼液的疗效。分别使用干燥应激和氢氧化钠在C57BL/6小鼠中诱导EDE和碱烧伤模型。在EDE和碱烧伤模型中，治疗组接受CMC、HPBS或HPE滴眼液。在EDE模型中，测量泪液体积、泪膜破裂时间（TBUT）和角膜荧光素总染色评分（CFSS）。用2'，7'-二氯二氢荧光素二乙酸酯检测活性氧（ROS）。通过过碘酸-希夫染色鉴定结膜杯状细胞，通过TUNEL法检测角膜上皮细胞凋亡。在碱烧伤模型中，评估每组上皮缺损的面积和直径。在EDE模型中，所有治疗组的泪液体积、CFSS和上皮细胞凋亡均有显著改善。与CMC组相比，HPE组在泪液体积产生、TBUT、CFSS、ROS和结膜杯状细胞密度方面有更好的改善。除14天时的TBUT外，HPBS组和HPE组之间的参数无显著差异。在碱烧伤模型中，与对照组和CMC组相比，HPE组的面积更小，与对照组相比直径更短。HPE滴眼液在改善EDE的临床体征和眼表氧化损伤以及促进碱烧伤后角膜上皮化方面与HPBS滴眼液一样有效。
