Li Zhengri, Cui Lian, Yang Jee Myung, Lee Hyo Seok, Choi Ji Suk, Woo Je Moon, Lim Seul Ki, Yoon Kyung Chul
a Xiamen Eye Center of Xiamen University , Xiamen , Fujian , China.
b Department of Ophthalmology , Chonnam National University Medical School and Hospital and Center for Creative Biomedical Scientists at Chonnam National University , Gwangju , South Korea.
Curr Eye Res. 2016 Nov;41(11):1424-1432. doi: 10.3109/02713683.2015.1133834. Epub 2016 May 9.
To investigate the wound healing effect of adiponectin eye drops following corneal alkali burn.
A chemical burn was induced using 0.1 M NaOH in both adenovirus 12-SV40 hybrid-transformed human corneal epithelial (HCE-2) cells and C57BL/6 mice. The injured HCE-2 and mice were then treated using either 0.1% hyaluronic acid (HA) or adiponectin at 0.0001%, 0.001%, or 0.01% concentration. The viability of the HCE-2 cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The wound healing rate of the HCE-2 cells was evaluated using a migration assay 4, 8, 12, 24, and 48 h after chemical injury. In mice, corneal epithelial defects and degree of haze were analyzed 6 h and 1, 2, 3, 6, and 7 days after chemical injury. Seven days after injury, the concentrations of IL-1β and transforming growth factor-β (TGF-β) in the cornea were measured using enzyme-linked immunosorbent assay, and histological analysis was also performed.
The viability of HCE-2 cells was not affected by adiponectin at any of the concentrations used. In HCE-2 cells treated using either 0.001% or 0.01% adiponectin, the wound healing rate after 4 h was significantly faster than in the control and HA-treated groups. With regard to mice, the 0.001% and 0.01% adiponectin-treated groups showed a significant improvement in epithelial defect parameters and haze scores at 3, 5, and 7 days after chemical injury. A significant decrease in IL-1β and TGF-β levels was observed in the 0.001% and 0.01% adiponectin-treated groups compared to the other groups. Histologically, corneal thickness and the inflammatory cells were also decreased in the adiponectin-treated groups.
Topical adiponectin (both 0.001% and 0.01%) increased epithelial migration and improved clinical signs and inflammation on the ocular surface after alkali burn, suggesting that adiponectin can promote wound healing in the cornea.
研究脂联素滴眼液对角膜碱烧伤后的伤口愈合作用。
在腺病毒12 - SV40杂交转化的人角膜上皮（HCE - 2）细胞和C57BL/6小鼠中，使用0.1 M氢氧化钠诱导化学烧伤。然后，对受伤的HCE - 2细胞和小鼠分别用0.1%透明质酸（HA）或浓度为0.0001%、0.001%或0.01%的脂联素进行处理。使用3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐法测定HCE - 2细胞的活力。在化学损伤后4、8、12、24和48小时，使用迁移试验评估HCE - 2细胞的伤口愈合率。在小鼠中，于化学损伤后6小时以及1、2、3、6和7天分析角膜上皮缺损情况和混浊程度。损伤7天后，使用酶联免疫吸附测定法测量角膜中白细胞介素 - 1β（IL - 1β）和转化生长因子 - β（TGF - β）的浓度，并进行组织学分析。
在所使用的任何浓度下，脂联素均未影响HCE - 2细胞的活力。在用0.001%或0.01%脂联素处理的HCE - 2细胞中，4小时后的伤口愈合率明显快于对照组和HA处理组。对于小鼠，在化学损伤后3、5和7天，0.001%和0.01%脂联素处理组的上皮缺损参数和混浊评分有显著改善。与其他组相比，0.001%和0.01%脂联素处理组的IL - 1β和TGF - β水平显著降低。组织学上，脂联素处理组的角膜厚度和炎症细胞也减少。
局部应用脂联素（0.001%和0.01%）可增加上皮迁移，并改善碱烧伤后眼表的临床体征和炎症，表明脂联素可促进角膜伤口愈合。
