Li Hongkun, Cai Qianqian, Bai Minghao, Jie Guifen
Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China.
Anal Chem. 2025 Jan 14;97(1):953-961. doi: 10.1021/acs.analchem.4c05678. Epub 2024 Dec 24.
The classical electrochemiluminescence (ECL) reagent Ru(bpy) was first doped into CdSe QDs to prepare novel dual-potential color-resolved luminophore Ru-CdSe QDs. Ru-CdSe QDs emitted a strong red ECL signal at a positive potential with coreactant TPrA and a strong green ECL signal at a negative potential with coreactant KSO. As a proof-of-concept application, this work introduced Ru-CdSe QDs into a dual-channel closed bipolar electrode (CBPE) system to construct an ECL biosensor for simultaneous detection of chloramphenicol (CAP) and kanamycin (KAN). Ru-CdSe QDs were dropped on BPE holes A and B for ECL emission. Cobalt single-atom catalysts (Co-N-C SACs) had superior electric double layer (EDL) performance and conductivity. It could greatly promote the electron transfer of the CBPE system and realize ECL signal amplification. Based on this characteristic, Co-N-C SACs were introduced into BPE hole C and driving electrode hole D, respectively, using the CAP and KAN split dual-aptamer sandwich strategy. During positive potential scanning, the polarity of BPE hole A was anode, and Ru-CdSe QDs emitted a red ECL signal. With the increase of CAP concentration, abundant Co-N-C SACs were introduced to the electrode surface. The positive potential ECL signal was increased for CAP detection. During negative potential scanning, the polarity of the BPE hole B was cathode, and Ru-CdSe QDs emitted a green ECL signal for KAN detection. Finally, a zero-background spatial-potential color-resolved CBPE-ECL biosensor was developed for dual-mode detection of CAP and KAN. This work explored a novel ECL luminophore to construct a CBPE-ECL sensor, which greatly facilitated the development of the ECL assay.
经典的电化学发光（ECL）试剂Ru(bpy)首次被掺杂到CdSe量子点中，以制备新型双电位颜色分辨发光体Ru-CdSe量子点。Ru-CdSe量子点在正电位下与共反应剂TPrA发射出强烈的红色ECL信号，在负电位下与共反应剂KSO发射出强烈的绿色ECL信号。作为概念验证应用，这项工作将Ru-CdSe量子点引入双通道封闭双极电极（CBPE）系统，构建了一种用于同时检测氯霉素（CAP）和卡那霉素（KAN）的ECL生物传感器。Ru-CdSe量子点滴涂在BPE孔A和B上用于ECL发射。钴单原子催化剂（Co-N-C SACs）具有优异的双电层（EDL）性能和导电性。它可以极大地促进CBPE系统的电子转移并实现ECL信号放大。基于这一特性，采用CAP和KAN分裂双适体夹心策略，将Co-N-C SACs分别引入BPE孔C和驱动电极孔D。在正电位扫描期间，BPE孔A的极性为阳极，Ru-CdSe量子点发射出红色ECL信号。随着CAP浓度的增加，大量的Co-N-C SACs被引入到电极表面。用于CAP检测的正电位ECL信号增强。在负电位扫描期间，BPE孔B的极性为阴极，Ru-CdSe量子点发射出绿色ECL信号用于KAN检测。最终，开发了一种零背景空间电位颜色分辨的CBPE-ECL生物传感器用于CAP和KAN的双模检测。这项工作探索了一种新型的ECL发光体来构建CBPE-ECL传感器，这极大地促进了ECL分析方法的发展。
