Francis Praise Mary, Vinod Vivek, Kumar V Anil, Mohan C Gopi, Biswas Raja
Amrita School for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
Department of Microbiology, Amrita Institute of Medical Sciences and Research Center, Amrita Vishwa Vidyapeetham, Kochi 682041, India.
Lett Appl Microbiol. 2025 Jan 6;78(1). doi: 10.1093/lambio/ovae134.
Mycobacterium marinum is a slow growing Non-Tuberculosis Mycobacteria known to cause skin and subcutaneous tissue infections known as "fish tank granuloma" in humans. Treatment of M. marinum skin infections can last for several months or even years. Intravenous or intramuscular administration of Amikacin, a bactericidal inhibitor, in a multidrug regimen is a good therapeutic option in treating M. marinum infections. No topical hydrogels are currently available to treat M. marinum skin infections. We developed a 2.5% amikacin-loaded carbopol hydrogel containing the permeation enhancers 1% Tween-80 and 1% PEG (AMK-CPTP) for topical application to treat M. marinum skin infections. A molecular docking study of carbopol-Amikacin interaction predicted a stable structure and was confirmed with rheological characterization. The developed gel was found to be non-hemolytic, cytocompatible, and non-irritant. Sustained release of Amikacin from the AMK-CPTP hydrogel was observed, with a release of 60% Amikacin after 48 h. The antibacterial activity and skin permeability of amikacin released from AMK-CPTP were assessed using both in vitro and ex vivo assays. Overall. AMK-CPTP demonstrated anti-microbial activity against M. marinum with a 35% increase in skin permeation on the addition of Tween-80 and PEG. AMK-CPTP could be used to treat M. marinum skin infections.
海分枝杆菌是一种生长缓慢的非结核分枝杆菌，已知可引起人类皮肤和皮下组织感染，即“鱼缸肉芽肿”。海分枝杆菌皮肤感染的治疗可能持续数月甚至数年。在多药治疗方案中静脉注射或肌肉注射杀菌抑制剂阿米卡星是治疗海分枝杆菌感染的一种良好治疗选择。目前尚无局部水凝胶可用于治疗海分枝杆菌皮肤感染。我们开发了一种含2.5%阿米卡星的卡波姆水凝胶，其中含有1%吐温80和1%聚乙二醇（AMK-CPTP）作为渗透促进剂，用于局部治疗海分枝杆菌皮肤感染。卡波姆-阿米卡星相互作用的分子对接研究预测了一种稳定结构，并通过流变学表征得到证实。所开发的凝胶被发现无溶血作用、具有细胞相容性且无刺激性。观察到阿米卡星从AMK-CPTP水凝胶中持续释放，48小时后释放60%的阿米卡星。使用体外和离体试验评估了从AMK-CPTP释放的阿米卡星的抗菌活性和皮肤渗透性。总体而言，AMK-CPTP对海分枝杆菌具有抗菌活性，添加吐温80和聚乙二醇后皮肤渗透性增加35%。AMK-CPTP可用于治疗海分枝杆菌皮肤感染。
