Ensing G J, Halie M R
Exp Hematol. 1985 Jan;13(1):1-6.
In order to follow monocyte kinetics in hematologic malignancies by means of radioactive labeling, three conditions are necessary: (a) no loss of monocytes during separation, (b) specific labeling of monocytes, and (c) normal functional capacities of the labeled monocytes. In this report a method is described that fulfills the first two conditions and can be executed with maintenance of sterility. Cell labeling was performed using a mononuclear cell suspension consisting of monocytes (20%-50%), lymphocytes (50%-80%), and with minor contamination by granulocytes, thrombocytes, and erythrocytes. This cell suspension was obtained by centrifugation of a leukocyte suspension on a gradient of 16% wt/vol and 22% wt/vol human serum albumin solutions. The recovery of monocytes with this enrichment method approached 100%. Monocytes were labeled by endocytosis of 111In-Fe colloid; monocytes were labeled significantly higher than lymphocytes (P less than 0.001) and granulocytes (P less than 0.01) (Wilcoxon's two-sample test). Cell viability after labeling was greater than 90%.
为了通过放射性标记追踪血液系统恶性肿瘤中的单核细胞动力学，需要满足三个条件：(a) 分离过程中单核细胞无损失，(b) 单核细胞特异性标记，以及 (c) 标记单核细胞的正常功能能力。在本报告中，描述了一种满足前两个条件且可在保持无菌的情况下执行的方法。使用由单核细胞（20%-50%）、淋巴细胞（50%-80%）组成，并受到粒细胞、血小板和红细胞轻微污染的单核细胞悬液进行细胞标记。该细胞悬液通过在16%重量/体积和22%重量/体积的人血清白蛋白溶液梯度上离心白细胞悬液获得。采用这种富集方法，单核细胞的回收率接近100%。通过111In-铁胶体的内吞作用标记单核细胞；单核细胞的标记率显著高于淋巴细胞（P<0.001）和粒细胞（P<0.01）（Wilcoxon双样本检验）。标记后细胞活力大于90%。
