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用于改善雪茄烟叶发酵效果的菌株分离与评估

Isolation and evaluation of strains to improve cigar tobacco leaves fermentation effect.

作者信息

Guo Sida, Li Yasen, Yang Zhen, Zhang Qianying, Li Pinhe, Jiang Zhongrong, Zhang Jiabao, Cao Yu, Zhang Zhengcheng, Li Dongliang

机构信息

Cigar Fermentation Technology Key Laboratory of China Tobacco, China Tobacco Sichuan Industrial Co., Ltd., Chengdu, China.

Industry Efficient Utilization of Domestic Cigar Tobacco Key Laboratory of Sichuan Province, China Tobacco Sichuan Industrial Co., Ltd., Shifang, China.

出版信息

Front Microbiol. 2024 Dec 10;15:1492042. doi: 10.3389/fmicb.2024.1492042. eCollection 2024.

Abstract

INTRODUCTION

Fermentation is a crucial process in cigar manufacturing. Exogenous addition of functional microorganisms for fortified fermentation can further improve cigar tobacco leaves fermentation quality.

METHODS

In this study, five strains were isolated from cigar tobacco leaves. Their fermentation effects were assessed by analyzing flavor components and major chemical compositions.

RESULTS

Among these five isolates, four strains demonstrated the ability to enhance the overall flavor profile, particularly by increasing esters and chlorophyll degradation products. Additionally, several strains, particularly F3 and F4, also reduced total alkaloid and nitrogen content. Among these, strain F4 exhibited the most significant improvements. Sensory evaluation revealed that strain F4 could enhance mellowness, richness and sweetness while reducing irritation and off-flavors in fermented cigar tobacco leaves.

DISCUSSION

Our findings indicate that strain F4 can significantly improve the overall quality of cigar tobacco leaves through fermentation. This study provides a promising microbial fermentation agent for potential application in the cigar industry.

摘要

引言

发酵是雪茄制造中的关键过程。外源添加功能性微生物进行强化发酵可进一步提高雪茄烟叶的发酵质量。

方法

本研究从雪茄烟叶中分离出五株菌株。通过分析风味成分和主要化学成分来评估它们的发酵效果。

结果

在这五株分离菌株中,四株表现出增强整体风味的能力,特别是通过增加酯类和叶绿素降解产物。此外,几株菌株,特别是F3和F4,还降低了总生物碱和氮含量。其中,菌株F4表现出最显著的改善。感官评价表明,菌株F4可增强发酵雪茄烟叶的醇厚感、丰富度和甜度,同时减少刺激性和异味。

讨论

我们的研究结果表明,菌株F4可通过发酵显著提高雪茄烟叶的整体质量。本研究为雪茄行业潜在应用提供了一种有前景的微生物发酵剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/53e6/11666510/317280ca6b4c/fmicb-15-1492042-g001.jpg

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