纯化免疫球蛋白G以测量人血清中碘化甲状腺球蛋白肽3,5,3',5'-四碘-L-酪氨酰-L-酪氨酸的水平。
IgG purification to measure the level of an iodinated thyroglobulin peptide, the 3,5,3',5' tetraiodo-l-tyrosyl-l-tyrosine in human serum.
作者信息
Bernard D, Maurizis J C, Nicolas C, Michelot J, Bétail G, Meyniel G
出版信息
Experientia. 1985 Mar 15;41(3):385-7. doi: 10.1007/BF02004519.
PMID:3972086
Abstract
Antibodies reacting with 3,5,3',5' tetraiodo-l-tyrosyl-l-tyrosine (I2Tyr-I2Tyr) were elicited in rabbits by immunization with an oxidized yeast conjugate coupled with I2Tyr-I2Tyr. Ion-exchange chromatography was used to purify immunoglobulins, in order to improve the specificity in measurement of I2Tyr-I2Tyr level in patient serum. IgG binding capacity versus I2Tyr-I2Tyr was considerably increased after immunoglobulin purification.
摘要
通过用与3,5,3',5'-四碘-L-酪氨酰-L-酪氨酸（I2Tyr-I2Tyr）偶联的氧化酵母缀合物免疫兔子，产生了与I2Tyr-I2Tyr反应的抗体。为了提高患者血清中I2Tyr-I2Tyr水平测量的特异性，使用离子交换色谱法纯化免疫球蛋白。免疫球蛋白纯化后，IgG与I2Tyr-I2Tyr的结合能力显著增加。
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