人胎盘合体滋养层细胞膜的分离与鉴定
Isolation and characterization of syncytiotrophoblast plasma membrane from human placenta.
作者信息
Khalfoun B, Degenne D, Arbeille-Brassart B, Gutman N, Bardos P
出版信息
FEBS Lett. 1985 Feb 11;181(1):33-8. doi: 10.1016/0014-5793(85)81108-x.
PMID:3972108
Abstract
Human full-term syncytiotrophoblast plasma membranes isolated by mechanical procedures (sieving and ultrasonic disintegration), purified by phase centrifugation, form a single band of 1.052 +/- 0.002 g/ml density in percoll gradient. The purity of the preparation was assessed by electron microscopy, enzyme analysis and beta 2-microglobulin determination.
摘要
通过机械方法（筛分和超声破碎）分离、经相离心纯化的人足月合体滋养层细胞膜，在 Percoll 梯度中形成一条密度为 1.052±0.002 g/ml 的单一带。通过电子显微镜、酶分析和β2-微球蛋白测定来评估制剂的纯度。
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