Vasconcelos Marina Wust, Vieira Dada Julia Morgana, Pereira Vitória Alves, Zandi-Karimi Ali, de Castilhos Ghisi Nédia, Oliveira De Barros Flavia Regina
Graduate Program in Genetics - Universidade Federal Do Paraná, Curitiba, Paraná, Brazil.
Graduate Program in Animal Science (PPZ) - Unioeste/Universidade Tecnológica Federal Do Paraná, Dois Vizinhos, Paraná, Brazil.
J Therm Biol. 2025 Jan;127:104028. doi: 10.1016/j.jtherbio.2024.104028. Epub 2024 Dec 20.
Heat stress can alter the expression of genes in the individual's molecular response. The identification of these genes makes it possible to better understand the molecular response, identifying biomarker genes and indirect response pathways that can help with genetic improvement studies, animal welfare, separating more thermotolerant varieties and mitigating the effects of heat stress. The aim of this scientometric review was to characterize the state of the art of scientific research into gene expression in ruminants under heat stress, to define the most studied species, biology systems and genes, as well as the related biological pathways and processes. The articles for the dataset were compiled in the Web of Science database, refined individually and analyzed using the CiteSpace, RStudio, Excel and GraphPad Prism programs and the KEGG (Kyoto Encyclopedia of Genes and Genomes) database. The publications formed a data set containing 271 articles and an H-index of 37. The number of publications increased from 2011. The countries with the highest frequency of publications are India, the United States, China and Brazil, the ruminant species are cattle, buffaloes, sheep and goats, all zootechnical interest, and biology systems was reproduction, blood and lactation, due to the economic importance of the quality and quantity of production, to the ease of collecting and possibility of studies in vitro. Cattle have been extensively studied in comparison to other ruminants. The HSP70 gene has been the most studied, followed by the HSP family, HSF, BAX, TLR and BCL-2, these genes can be molecular markers of heat stress. The main pathways and biological processes of genes were in cattle the cancer pathway; in goats the Mixed, incl. myd88-dependent toll-like receptor signaling pathway, and lipopolys; in sheep the oxidoreductase; and in buffalo it was the BCL-2 family. The molecular responses are still recent and have not been established.
热应激可改变个体分子反应中基因的表达。对这些基因的识别有助于更好地理解分子反应，识别生物标志物基因和间接反应途径，这有助于开展遗传改良研究、动物福利研究、筛选耐热性更强的品种以及减轻热应激的影响。本科学计量学综述的目的是描述热应激下反刍动物基因表达的科学研究现状，确定研究最多的物种、生物学系统和基因，以及相关的生物学途径和过程。数据集中的文章是在科学网数据库中收集的，经过单独筛选，并使用CiteSpace、RStudio、Excel和GraphPad Prism程序以及KEGG（京都基因与基因组百科全书）数据库进行分析。这些出版物形成了一个包含271篇文章且H指数为37的数据集。自2011年以来，出版物数量不断增加。出版物频率最高的国家是印度、美国、中国和巴西，反刍动物物种是牛、水牛、绵羊和山羊，这些都具有畜牧学研究价值，生物学系统是繁殖、血液和泌乳，这是由于生产质量和数量的经济重要性、样本采集的便利性以及体外研究的可能性。与其他反刍动物相比，牛的研究最为广泛。热休克蛋白70（HSP70）基因是研究最多的基因，其次是热休克蛋白（HSP）家族、热休克因子（HSF）、促凋亡蛋白BAX、Toll样受体（TLR）和凋亡抑制蛋白BCL-2，这些基因可能是热应激的分子标志物。基因的主要途径和生物学过程在牛中是癌症途径；在山羊中是混合途径，包括髓样分化因子88（myd88）依赖的Toll样受体信号通路和脂多糖；在绵羊中是氧化还原酶；在水牛中是BCL-2家族。分子反应研究仍处于起步阶段，尚未完全确立。
