Shen Qi, Ding Jianjun, Guo Zengsheng, Wang Chenchen, Zhang Yuhan, Lin Cuiping, Sun Yiqiang, Hang Lifeng
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250055, PR China.
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250055, PR China.
Colloids Surf B Biointerfaces. 2025 Apr;248:114471. doi: 10.1016/j.colsurfb.2024.114471. Epub 2024 Dec 22.
Alpha-fetoprotein (AFP), serves as a reliable and vital biomarker for precise diagnosis and effective monitoring of hepatocellular carcinoma, requires precise detection. Herein, a sandwich-structured electrochemical immunosensor was crafted, employing three-dimensional layered porous carbon modified with gold nanoparticles (Au NPs) as the substrate and Au NPs/CuS as the labeling compound for accurate and sensitive detection of AFP. Due to the effective coordination between the 3D carbon network, Au NPs, and hollow CuS nanocubes, the sandwich-structured electrochemical immunosensor was able to produce three distinct response signals via various detection techniques, demonstrating a broad linear range (0.0001-400 ng/mL), exceptional sensitivity, and a remarkably low detection limit of 2.63 fg/mL. Moreover, the constructed immunosensor could be used to detect AFP in human serum. This research may offer a novel material framework for developing highly sensitive immunosensors.
甲胎蛋白（AFP）是用于肝细胞癌精确诊断和有效监测的可靠且重要的生物标志物，需要进行精确检测。在此，构建了一种夹心结构的电化学免疫传感器，采用金纳米颗粒（Au NPs）修饰的三维层状多孔碳作为基底，以及Au NPs/CuS作为标记化合物，用于准确、灵敏地检测AFP。由于三维碳网络、Au NPs和中空CuS纳米立方体之间的有效配位，该夹心结构的电化学免疫传感器能够通过各种检测技术产生三种不同的响应信号，显示出较宽的线性范围（0.0001 - 400 ng/mL）、出色的灵敏度以及低至2.63 fg/mL的检测限。此外，构建的免疫传感器可用于检测人血清中的AFP。该研究可能为开发高灵敏度免疫传感器提供一种新型材料框架。
