Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of 8 Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Biosens Bioelectron. 2013 Nov 15;49:222-5. doi: 10.1016/j.bios.2013.05.016. Epub 2013 May 25.
A novel electrochemical immunosensor capable of enzyme-free detection of alpha fetoprotein (AFP) is reported. This immunosensor was fabricated in a sandwich-like format where catalytic Au-Pd nanocrystals and highly conductive N-doped graphene sheets were incorporated. The significant catalysis by Au-Pd nanocrystals toward hydrogen peroxide, along with the increased electron transfer by graphene sheets, caused signal generation and increased sensitivity, which enables the enzyme-free detection of AFP. With a low detection limit at 0.005 ng mL(-1), this novel immunosensor worked well over the broad linear range of 0.05-30 ng mL(-1). Unlike previously reported enzyme-based electrochemical immunosensors, which often involve the complicated steps for enzyme loading and necessary treatments to keep the activity of enzyme, this novel immunosensor is simple in nature and employed catalytic Au-Pd nanoparticles and highly conductive graphene, which thus enables reliable and sensitive detection for clinic usage.
一种新型的电化学无酶免疫传感器可用于检测甲胎蛋白（AFP）。该免疫传感器采用三明治结构，其中包含了具有催化作用的金钯纳米晶和高导电性的氮掺杂石墨烯片。金钯纳米晶对过氧化氢具有显著的催化作用，同时石墨烯片增加了电子转移，从而产生信号并提高了灵敏度，实现了无酶 AFP 的检测。该新型免疫传感器具有较低的检测下限，为 0.005ng/mL，在 0.05-30ng/mL 的宽线性范围内具有良好的工作性能。与之前报道的基于酶的电化学免疫传感器不同，后者通常涉及酶负载的复杂步骤和保持酶活性的必要处理，这种新型免疫传感器具有简单的性质，采用了具有催化作用的金钯纳米颗粒和高导电性的石墨烯，因此能够实现可靠和灵敏的临床检测。
