Zhang Wen, Munyaneza Venuste, Wang Dandan, Huang Chenfeng, Wu Siyuan, Han Mingcun, Wang Xu, Kant Surya, Ding Guangda
College of Resources and Environment/Microelement Research Center/Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, 430070, Wuhan, PR China.
Institute of Quality Standard and Monitoring Technology for Agro-products of Guangdong Academy of Agricultural Sciences, Key Laboratory of Testing and Evaluation for Agro-product Safety and Quality, Ministry of Agriculture and Rural Affairs, PR China.
J Plant Physiol. 2025 Jan;304:154411. doi: 10.1016/j.jplph.2024.154411. Epub 2024 Dec 16.
Nitrogen (N) is crucial for plant growth, available primarily as nitrate (NO) and ammonium (NH). However, its presence in soil is often limited, necessitating strategies to augment N availability. This study delves into the enigmatic interplay between NO and NH in fostering the growth of Brassica napus, an important oil crop worldwide. Here, we examined the growth responses of 49 B. napus varieties to five NH:NO ratios (12:0, 9:3, 3:9, 1:11, 0:12). In general, the biomass of 49 rapeseed varieties increased with the decrease of NH to NO ratios in the growth environment. However, different varieties may respond diversely to the mixed N sources, or sole NO or NH condition. For some cultivars, the mixed N supply significantly enhanced the plant growth compared with the sole NO conditions. Thus, we further investigate the morphological, physiological and molecular response of rapeseed to the mixed N source condition using sole NO as a control. The results show that partial replacement by ammonium nutrition in the environment can promote rapeseed root development, net photosynthetic rate and NO reduction compared to NO-only conditions. Using transcriptome analysis, we found a total of 399 and 465 genes which were differentially expressed in root and shoot under A1N11 compared to A0N12 treatments, respectively. Genes involved in photosynthesis, N uptake and assimilation were upregulated by mixed N supplies. These findings highlight that the mixed N supply primarily stimulates B. napus growth by enhancing root development, photosynthesis and N metabolism in the shoot. Such insights are crucial for optimizing N form selection in B. napus to enhance plant performance and N use efficiency.
氮（N）对植物生长至关重要，主要以硝酸盐（NO）和铵盐（NH）的形式存在。然而，其在土壤中的含量往往有限，因此需要采取策略来提高氮的有效性。本研究深入探讨了NO和NH在促进全球重要油料作物甘蓝型油菜生长过程中神秘的相互作用。在此，我们研究了49个甘蓝型油菜品种对五种NH:NO比例（12:0、9:3、3:9、1:11、0:12）的生长反应。总体而言，49个油菜品种的生物量随着生长环境中NH与NO比例的降低而增加。然而，不同品种对混合氮源、单一NO或NH条件的反应可能不同。对于一些品种，与单一NO条件相比，混合氮供应显著促进了植物生长。因此，我们以单一NO为对照，进一步研究了油菜对混合氮源条件的形态、生理和分子反应。结果表明，与仅供应NO的条件相比，环境中铵态氮营养的部分替代可促进油菜根系发育、净光合速率和NO还原。通过转录组分析，我们发现与A0N12处理相比，在A1N11处理下，根和地上部分别有399个和465个基因差异表达。参与光合作用、氮吸收和同化的基因在混合氮供应下上调。这些发现突出表明，混合氮供应主要通过增强根系发育、光合作用和地上部氮代谢来刺激甘蓝型油菜生长。这些见解对于优化甘蓝型油菜的氮形态选择以提高植物性能和氮利用效率至关重要。
