Szopa T M, Rousseaux J J, Yuncker C, Happ G M
Dev Biol. 1985 Feb;107(2):325-36. doi: 10.1016/0012-1606(85)90315-x.
During the 9-day pupal period of Tenebrio molitor (the mealworm beetle), the cells of the male accessory glands undergo divisions for 7 days. There are two maxima in the mitotic activity in the glands in vivo, one at 1 day and the other at 4 days. The latter peak coincides with the large surge of ecdysterone occurring in the pupal stage. By the use of in vitro culture techniques, it has been demonstrated that the first bout of mitosis in both glands proceeds in basal medium, while the second bout of mitosis requires a physiological level of ecdysterone. Ecdysone was less effective than ecdysterone. Sensitivity to ecdysterone did not change significantly between Day 1 and Day 4 of the pupal stage. The results are discussed in relation to the effects of ecdysterone on cell division in mesodermal and ectodermal derivatives.
在黄粉虫的9天蛹期，雄性附腺细胞会进行7天的分裂。在活体状态下，腺体中的有丝分裂活性有两个高峰期，一个在第1天，另一个在第4天。后一个峰值与蛹期出现的蜕皮甾酮大量激增相吻合。通过体外培养技术已经证明，两个腺体的第一轮有丝分裂在基础培养基中进行，而第二轮有丝分裂则需要生理水平的蜕皮甾酮。蜕皮激素的效果不如蜕皮甾酮。在蛹期的第1天到第4天之间，对蜕皮甾酮的敏感性没有显著变化。结合蜕皮甾酮对中胚层和外胚层衍生物细胞分裂的影响对结果进行了讨论。
