Zheng Zixuan, Ma Xueqing, Li Kun, Sun Pu, Huang Shulun, Dong Kaiheng, Zhao Qiongqiong, Lu Zengjun, Qian Ping
National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
State Key Laboratory for Animal Disease Control and Prevention, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Dec 25;40(12):4493-4508. doi: 10.13345/j.cjb.240084.
The aim of this study was to compare the immune responses of C57BL/6 mice immunized with two pathogens, foot-and-mouth disease virus (FMDV) and Senecavirus A (SVA), and to provide clues for revealing the regulatory mechanisms of acquired immunity. Inactivated and purified FMDV and SVA antigens were used to immunize C57BL/6 mice respectively, and the mice immunized with PBS were taken as the control. The percentages of Th1 and Th2 cells in the spleen lymphocytes of mice in each group were analyzed by flow cytometry at 14 and 28 days after immunization. RNA-Seq was performed for the spleen. Mouse macrophages were stimulated with the antigens to examine the expression of the differentially expressed genes (DEGs) screened out. The results showed that 14 days after immunization, there was no significant difference in the magnitude of the Th1/Th2 immune response elicited by the FMDV and SVA antigens. After 28 days, the magnitudes of the Th1 and Th2 immune responses elicited by the SVA antigen were higher than those elicited by the FMDV antigen. RNA-Seq revealed two common DEGs, and , between the two immunization groups, which indicated that the two genes may be involved in the activation of the Th1/Th2 immune responses by FMDV and SVA antigens. FMDV and SVA antigens stimulated macrophages to secrete interleukin (IL)-12 and IL-33 , and the expression of and was consistent with the RNA-Seq results. The expression of was regulated by type I interferons (IFNα, IFNβ). In this study, we obtained the DEGs involved in the immune responses to the two antigens in mouse spleen, which provides a molecular basis for investigating the immune response mechanisms induced by FMDV and SVA.
本研究旨在比较用口蹄疫病毒（FMDV）和塞内卡病毒A（SVA）这两种病原体免疫的C57BL/6小鼠的免疫反应，为揭示获得性免疫的调节机制提供线索。分别用灭活和纯化的FMDV和SVA抗原免疫C57BL/6小鼠，并将用PBS免疫的小鼠作为对照。在免疫后14天和28天，通过流式细胞术分析每组小鼠脾淋巴细胞中Th1和Th2细胞的百分比。对脾脏进行RNA测序。用抗原刺激小鼠巨噬细胞，以检测筛选出的差异表达基因（DEG）的表达情况。结果显示，免疫后14天，FMDV和SVA抗原引发的Th1/Th2免疫反应强度无显著差异。28天后，SVA抗原引发的Th1和Th2免疫反应强度高于FMDV抗原引发的反应强度。RNA测序揭示了两个免疫组之间的两个共同DEG，表明这两个基因可能参与FMDV和SVA抗原对Th1/Th2免疫反应的激活。FMDV和SVA抗原刺激巨噬细胞分泌白细胞介素（IL）-12和IL-33，且 和 的表达与RNA测序结果一致。 的表达受I型干扰素（IFNα、IFNβ）调节。在本研究中，我们获得了小鼠脾脏中参与对这两种抗原免疫反应的DEG，为研究FMDV和SVA诱导的免疫反应机制提供了分子基础。
