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乳酸菌表面蛋白在细胞黏附与免疫调节机制中的作用

Lactic Acid Bacteria Surface Proteins in the Mechanisms of Cell Adhesion and Immunoregulation.

作者信息

Meng Ziheng, Huang Xianqing, Qiao Mingwu, Song Lianjun, Liu Yufei, Hai Dan

机构信息

College of Food Science and Technology Henan Agricultural University Zhengzhou China.

Henan Engineering Technology Research Center of Food Processing and Circulation Safety Control Zhengzhou China.

出版信息

Food Sci Nutr. 2024 Oct 30;12(12):10148-10163. doi: 10.1002/fsn3.4517. eCollection 2024 Dec.

DOI:10.1002/fsn3.4517
PMID:39723039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11666997/
Abstract

This study delves into the role of lactic acid bacteria (LAB) surface proteins in cell adhesion and immunoregulation. Using fluorescence microscopy, we observed distinct adhesion patterns on various cell types. LAB surface proteins demonstrated concentration-dependent inhibition of Salmonella adhesion, with LAB69 exhibiting potent antagonistic effects. Genetic expression analysis revealed nuanced responses in key genes (MD2, TLR4, IL-10, MUC3, MIF) across different cell types, highlighting the diverse immunomodulatory effects of LAB surface proteins. Modulation of pro-inflammatory (TNF-α) and anti-inflammatory (IL-10) cytokines further emphasized the complex interplay. In conclusion, this study underscores the pivotal role of LAB surface proteins in mediating cell adhesion and immunoregulation, providing a foundation for isolating specific immunomodulatory molecules within LAB surface proteins for potential applications in microbial ecological agents.

摘要

本研究深入探讨了乳酸菌（LAB）表面蛋白在细胞黏附和免疫调节中的作用。通过荧光显微镜观察，我们在多种细胞类型上发现了不同的黏附模式。LAB表面蛋白对沙门氏菌黏附表现出浓度依赖性抑制，其中LAB69表现出强大的拮抗作用。基因表达分析揭示了不同细胞类型中关键基因（MD2、TLR4、IL - 10、MUC3、MIF）的细微反应，突出了LAB表面蛋白多样的免疫调节作用。促炎细胞因子（TNF - α）和抗炎细胞因子（IL - 10）的调节进一步强调了这种复杂的相互作用。总之，本研究强调了LAB表面蛋白在介导细胞黏附和免疫调节中的关键作用，为从LAB表面蛋白中分离特定免疫调节分子以用于微生物生态制剂的潜在应用奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e7/11666997/8e1627f4f191/FSN3-12-10148-g016.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e7/11666997/9b9cab55c454/FSN3-12-10148-g018.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e7/11666997/8e1627f4f191/FSN3-12-10148-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e7/11666997/f2a71964b9a2/FSN3-12-10148-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e7/11666997/e1cbc722beb6/FSN3-12-10148-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e7/11666997/6e0408515d25/FSN3-12-10148-g017.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e7/11666997/037d326dfd08/FSN3-12-10148-g012.jpg
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本文引用的文献

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Surface (S) Layer Proteins of Block Virus Infection via DC-SIGN Interaction.表面（S）层蛋白通过与DC-SIGN相互作用阻断病毒感染。
Front Microbiol. 2019 Apr 16;10:810. doi: 10.3389/fmicb.2019.00810. eCollection 2019.
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Lactobacillus rhamnosus GG prevents epithelial barrier dysfunction induced by interferon-gamma and fecal supernatants from irritable bowel syndrome patients in human intestinal enteroids and colonoids.罗伊氏乳杆菌 GG 可预防肠上皮细胞屏障功能障碍，该作用由γ干扰素和肠易激综合征患者粪便上清液诱导，并在人肠类器官和结肠类器官中得到证实。
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Effects of dietary Lactobacillus rhamnosus CF supplementation on growth, meat quality, and microenvironment in specific pathogen-free chickens.
鼠李糖乳杆菌 CF 膳食补充对无特定病原体鸡生长、肉质和微环境的影响。
Poult Sci. 2018 Jan 1;97(1):118-123. doi: 10.3382/ps/pex261.
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Functional Analysis of an S-Layer-Associated Fibronectin-Binding Protein in Lactobacillus acidophilus NCFM.嗜酸乳杆菌NCFM中一种与S层相关的纤连蛋白结合蛋白的功能分析
Appl Environ Microbiol. 2016 Apr 18;82(9):2676-2685. doi: 10.1128/AEM.00024-16. Print 2016 May.
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Alterations of digestive enzyme activities, intestinal morphology and microbiota in juvenile paddlefish, Polyodon spathula, fed dietary probiotics.饲喂益生菌的匙吻鲟幼鱼消化酶活性、肠道形态和微生物群的变化
Fish Physiol Biochem. 2015 Feb;41(1):91-105. doi: 10.1007/s10695-014-0008-7. Epub 2014 Nov 18.
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Adhesive ability means inhibition activities for lactobacillus against pathogens and S-layer protein plays an important role in adhesion.黏附能力是指乳酸菌对病原体的抑制活性，而 S-层蛋白在黏附中起着重要作用。
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