Fang Cheng, Ma Mingyang, Ji Hong, Ren Tongjun, Mims Steven D
College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, People's Republic of China.
Fish Physiol Biochem. 2015 Feb;41(1):91-105. doi: 10.1007/s10695-014-0008-7. Epub 2014 Nov 18.
The effects of dietary supplementation of probiotics on digestive enzymes activities, intestinal morphology and microbiota in juvenile paddlefish (Polyodon spathula) were studied. A total of 400 fish were reared in two cages and fed with a basal diet (control group, CG) or diet supplemented with commercial probiotics (treatment group, TG) for 80 days. Enzymes activities analysis indicated that protease and α-amylase activities increased (P < 0.01 or P < 0.05) in TG. Light microscopy observation demonstrated the decrease of wall thickness and muscularis thickness in foregut (P < 0.01), the increase of those in hindgut (P < 0.05), the increase of folds height in foregut (P < 0.01) and midgut in TG (P < 0.05). DGGE results of PCR-amplified 16S rRNA confirmed that the richness and diversity of intestinal microbial species increased in TG. The similarity between the commercial bacteria product and intestinal microbiota of TG were higher than the microbiota from CG. The quantities of bacterium, Firmicutes, Proteobacteria, Bacteroidetes, Fusobacteria, present an increasing trend from foregut to hindgut both in two groups. To our knowledge, this is the first in vivo study to reveal the effect of dietary probiotics on intestinal digestive enzymes activities, morphology and microbiota in paddlefish.
研究了日粮添加益生菌对匙吻鲟幼鱼消化酶活性、肠道形态和微生物群的影响。将400尾鱼饲养在两个网箱中，分别投喂基础日粮（对照组，CG）或添加商业益生菌的日粮（处理组，TG），持续80天。酶活性分析表明，处理组的蛋白酶和α-淀粉酶活性增加（P<0.01或P<0.05）。光学显微镜观察显示，处理组前肠壁厚度和肌层厚度降低（P<0.01），后肠壁厚度和肌层厚度增加（P<0.05），前肠褶皱高度增加（P<0.01），中肠褶皱高度增加（P<0.05）。PCR扩增16S rRNA的DGGE结果证实，处理组肠道微生物种类的丰富度和多样性增加。处理组商业细菌产品与肠道微生物群之间的相似性高于对照组微生物群。两组中，从前往后，厚壁菌门、变形菌门、拟杆菌门、梭杆菌门的细菌数量均呈增加趋势。据我们所知，这是第一项揭示日粮益生菌对匙吻鲟肠道消化酶活性、形态和微生物群影响的体内研究。
