Hajimohammadi Mahdi, Sheikh Mahboobi Fatemeh, Wu Haizhou
Faculty of Chemistry Kharazmi University Tehran Iran.
College of Food Science and Technology Huazhong Agricultural University Wuhan China.
Food Sci Nutr. 2024 Nov 11;12(12):10405-10411. doi: 10.1002/fsn3.4539. eCollection 2024 Dec.
Photosensitizers and pigments in raw meat such as porphyrins, riboflavin, and myoglobin after incorporation with light beam prompt the generation of singlet oxygen (O) from triplet oxygen (O) and cause oxidative rancidity of meat products. In this study, the results of photooxidation reactions of sheep erythrocyte (red blood cell) model as a model rich in hemoglobin and phospholipids bilayer, and oleic acid model were obtained by H NMR spectroscopy, TBARS assay, and iodometric titration. In both models, the rate of lipid photooxidation in the presence of hydroalcoholic extracts of Turmeric ( L.) and Cumin ( L.) as natural antioxidants, Butyl hydroxytoluene (BHT) as a synthetic antioxidant, and sodium azide (NaN) as a well-known O scavenger were decreased in the order of NaN > Turmeric > Cumin > BHT. It was proven that during the photooxidation process, there is a direct association between the amount of flavonoid compounds and O scavenging.
生肉中的光敏剂和色素,如卟啉、核黄素和肌红蛋白,在与光束结合后会促使三线态氧(O)生成单线态氧(O),并导致肉制品氧化酸败。在本研究中,通过核磁共振氢谱、硫代巴比妥酸反应物(TBARS)测定和碘量滴定法,获得了富含血红蛋白和磷脂双层的绵羊红细胞(红血球)模型以及油酸模型的光氧化反应结果。在这两种模型中,作为天然抗氧化剂的姜黄(L.)和孜然(L.)的水醇提取物、作为合成抗氧化剂的丁基羟基甲苯(BHT)以及作为著名的单线态氧清除剂的叠氮化钠(NaN)存在时,脂质光氧化速率按NaN>姜黄>孜然>BHT的顺序降低。结果证明,在光氧化过程中,黄酮类化合物的含量与单线态氧清除之间存在直接关联。