银纳米颗粒和齐留通通过铁死亡和减轻炎症对多囊卵巢综合征的协同作用。
Synergistic effects of AgNPs and zileuton on PCOS via ferroptosis and inflammation mitigation.
作者信息
Eltokhy Amira K, El-Shaer Rehab Ahmed Ahmed, El-Deeb Omnia Safwat, Farghal Eman E, Ibrahim Rowida Raafat, Elesawy Rasha, Awad Marwa Mahmoud, Ismail Radwa, Motawea Shaimaa M, Shatat Doaa, Hafez Yasser Mostafa, El Hanafy Hend Ahmed, Atef Marwa Mohamed
机构信息
Department of Medical Biochemistry, Tanta Faculty of Medicine, Tanta University, Tanta, Egypt.
Department of Medical Physiology, Tanta Faculty of Medicine, Tanta University, Tanta, Egypt.
出版信息
Redox Rep. 2025 Dec;30(1):2445398. doi: 10.1080/13510002.2024.2445398. Epub 2024 Dec 26.
BACKGROUND
The most prevalent endocrine disorder affecting women is PCOS. Programmed death of ovarian cells has yet to be elucidated. Ferroptosis is a kind of iron-dependent necrosis featured by significantly Fe-dependent lipid peroxidation. The ongoing study aimed to reinforce fertility by combining therapy with AgNPs and (Zileuton) in PCOS rats' model.
METHODS
The study included 75 adult female rats divided into 5 groups; control, PCOS, PCOS treated with AgNPs, PCOS treated with Zileuton, and PCOS group treated with AgNPs and Zileuton. The study investigated the anti-ferroptotic, anti-inflammatory, antioxidant, antiapoptotic, histopathological and immunohistochemical examinations of COX-2 and VEGF.
RESULTS
The combination of AgNPs and Zileuton showed significant reduction of inflammatory mediators (IL-6, TNF-α, NFk-B) compared with diseased group (- < 0.05), regression of ferroptosis marks (Panx1 and TLR4 expression, Fe levels) compared with diseased group (- < 0.05), depression of apoptotic marker caspase 3 level compared with diseased animals (-value < 0.05), depression of MDA level, elevation of HO-1, GPx4 activity, and reduction of Cox2 and VEGF as compared with the diseased, AgNPs or zileuton-treated groups (-value < 0.05).
CONCLUSION
The study showed that the combination of AgNPs and zileuton guards against, inflammation, apoptosis, and ferroptosis in PCO.
背景
影响女性的最常见内分泌疾病是多囊卵巢综合征（PCOS）。卵巢细胞的程序性死亡尚未阐明。铁死亡是一种铁依赖性坏死，其特征是显著的铁依赖性脂质过氧化。本研究旨在通过在PCOS大鼠模型中联合使用纳米银颗粒（AgNPs）和齐留通来提高生育能力。
方法
该研究包括75只成年雌性大鼠，分为5组；对照组、PCOS组、用AgNPs治疗的PCOS组、用齐留通治疗的PCOS组以及用AgNPs和齐留通治疗的PCOS组。该研究调查了COX-2和VEGF的抗铁死亡、抗炎、抗氧化、抗凋亡、组织病理学和免疫组化检查。
结果
与疾病组相比，AgNPs和齐留通联合使用显示炎症介质（IL-6、TNF-α、NFk-B）显著降低（-<0.05），与疾病组相比，铁死亡标志物（Panx1和TLR4表达、铁水平）消退（-<0.05），与患病动物相比，凋亡标志物半胱天冬酶3水平降低（-值<0.05），与患病、AgNPs或齐留通治疗组相比，丙二醛（MDA）水平降低，血红素加氧酶-1（HO-1）升高、谷胱甘肽过氧化物酶4（GPx4）活性升高，Cox2和VEGF降低（-值<0.05）。
结论
该研究表明，AgNPs和齐留通联合使用可预防PCO中的炎症、凋亡和铁死亡。
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