Sun Bo, Zhong Yuan, Tao Zhihuan, Zhu Lin, Miao Xuexia, Shi Zhenying, Li Haichao
Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, China.
ZJU-Hangzhou Global Scientific and Technological Innovation Centre, Zhejiang University, Hangzhou, 311200, China.
Plant Cell Rep. 2024 Dec 26;44(1):13. doi: 10.1007/s00299-024-03411-8.
OsMYB1 negatively mediates rice resistance to brown planthopper and rice blight. Additionally, OsMYB1 interacts with OsSPL14 and antagonizes its function by oppositely regulating downstream resistance-related genes. In their natural habitats, plants are concurrently attacked by different biotic factors. Xanthomonas oryzae pv. oryzae (Xoo) is a pathogen that severely deteriorates rice yield and quality, and brown planthopper (BPH; Nilaparvata lugens) is a rice specific insect pest with the damage topping other pathogens. Although genes for respective resistance to BPH and Xoo have been widely reported, few studies pay attention to simultaneous resistance to both. In this study, we identified a MYB transcription factor, OsMYB1, which exhibited diverse transcriptional regulatory capabilities and a negative regulatory role in resistance to both BPH and Xoo. Biochemical and genetic analysis proved OsMYB1 to be a TF that could interact with OsSPL14, a positive regulator of rice resistance to Xoo. OsSPL14 mutants showed increased sensitivity to BPH, suggesting that OsSPL14 is contrary to OsMYB1 in regulating rice resistance to these two biotic stresses. Consistently, OsMYB1 and OsSPL14 displayed opposite functions in regulating defense-related genes. OsMYB1 can form transcription regulation complexes with repressor OsJAZs instead of co-repressor TOPLESS to possibly realize its transcriptional repression function. Taken together, we concluded that two interacting TFs in rice, OsMYB1 and OsSPL14, played antagonistic roles in regulating resistance to BPH and Xoo.
OsMYB1负向介导水稻对褐飞虱和稻瘟病的抗性。此外，OsMYB1与OsSPL14相互作用，并通过反向调节下游抗性相关基因来拮抗其功能。在自然栖息地中，植物会同时受到不同生物因子的攻击。水稻白叶枯病菌（Xanthomonas oryzae pv. oryzae，Xoo）是一种严重降低水稻产量和品质的病原体，而褐飞虱（BPH；Nilaparvata lugens）是一种水稻特有的害虫，其危害超过其他病原体。虽然分别针对BPH和Xoo的抗性基因已有广泛报道，但很少有研究关注对两者的同时抗性。在本研究中，我们鉴定了一个MYB转录因子OsMYB1，它表现出多样的转录调控能力，并在对BPH和Xoo的抗性中发挥负调控作用。生化和遗传分析证明OsMYB1是一个能够与水稻对Xoo抗性的正向调节因子OsSPL14相互作用的转录因子。OsSPL14突变体对BPH表现出更高的敏感性，这表明OsSPL14在调节水稻对这两种生物胁迫的抗性方面与OsMYB1相反。一致地，OsMYB1和OsSPL14在调节防御相关基因方面表现出相反的功能。OsMYB1可以与阻遏蛋白OsJAZs形成转录调控复合物，而不是与共阻遏蛋白TOPLESS形成复合物，从而可能实现其转录抑制功能。综上所述，我们得出结论，水稻中的两个相互作用的转录因子OsMYB1和OsSPL14在调节对BPH和Xoo的抗性中发挥拮抗作用。
