Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, 350018, China.
College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
BMC Plant Biol. 2023 Jan 25;23(1):55. doi: 10.1186/s12870-023-04062-9.
Drought is a major abiotic stress to rice (Oryza sativa) during growth. Ideal Plant Architecture (IPA1), the first cloned gene controlling the ideal plant type in rice, has been reported to function in both ideal rice plant architecture and biotic resistance. Here, we report that the IPA1/OsSPL14, encoding a transcriptional factor, positively regulates drought tolerance in rice. The IPA1 is constitutively expressed and regulated by HO, abscisic acid, NaCl and polyethylene glycol 6000 treatments in rice. Furthermore, the IPA1-knockout plants showed much greater accumulation of HO as measured by 3,3'-diaminobenzidine staining in leaves compared with WT plants. Yeast one-hybrid, dual-luciferase and electrophoretic mobility shift assays indicated that the IPA1 directly activates the promoter of SNAC1. Expression of SNAC1 is significantly down-regulated in IPA1 knockout plants. Further investigation indicated that the IPA1 plays a positive role in drought-stress tolerance by inducing reactive oxygen species scavenging in rice. Together, these findings indicated that the IPA1 played important roles in drought tolerance by regulating SNAC1, thus activating the antioxidant system in rice.
干旱是水稻生长过程中的主要非生物胁迫。理想株型（IPA1）是第一个克隆的控制水稻理想株型的基因，已被报道在理想水稻株型和生物抗性中发挥作用。在这里，我们报告称，IPA1/OsSPL14 编码一个转录因子，可正向调控水稻的耐旱性。IPA1 在水稻中组成型表达，并受 HO、脱落酸、NaCl 和聚乙二醇 6000 处理的调节。此外，IPA1 敲除植物的 HO 积累量比 WT 植物高得多，这可以通过 3,3'-二氨基联苯胺染色来衡量。酵母单杂交、双荧光素酶报告基因和电泳迁移率变动分析表明，IPA1 直接激活 SNAC1 的启动子。SNAC1 在 IPA1 敲除植物中的表达明显下调。进一步的研究表明，IPA1 通过诱导水稻中活性氧的清除，在耐旱性方面发挥积极作用。综上所述，这些发现表明 IPA1 通过调节 SNAC1 来发挥重要作用，从而激活水稻中的抗氧化系统，进而提高水稻的耐旱性。
